Efficient organogenesis and plantlet regeneration in the timber species cunninghamia lanceolata (lamb.) hook
文献类型：期刊论文
| 作者 | Zhu, Mu-Lan; Wang, Ji; Yu, Yang; Liu, Sheng-Jun; Wei, Zhi-Ming |
| 刊名 | In vitro cellular & developmental biology-plant
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 43期号:5页码:449-455 |
| 关键词 | Cunninghamia lanceolata In vitro propagation Organogenesis Regeneration Timber biotechnology |
| ISSN号 | 1054-5476 |
| DOI | 10.1007/s11627-007-9092-z |
| 通讯作者 | Wei, zhi-ming(zmwei@sippe.ac.cn) |
| 英文摘要 | Two efficient regeneration systems were developed in cunninghamia lanceolata, the most important conifer for industrial wood production in china. cotyledons and hypocotyls derived from greenhouse-grown seedlings were used as initial explants in our research. a high frequency (95.1 +/- 1.84%) of adventitious buds were initiated directly from cotyledons cultured on douglas-fir cotyledon revised (dcr) medium supplemented with 1 mg l(-1) benzyladenine (ba), 0.1 mg l(-1) alpha-naphthaleneacetic acid (naa), and 0.004 mg l(-1) thidiazuron (tdz) with a maximum mean number of adventitious buds per cotyledon explant of 3.76 +/- 0.08. in contrast, a high percentage (93.73 +/- 0.55%) of adventitious buds regenerated via callus produced from hypocotyls cultured on dcr medium supplemented with plant growth regulators with a maximum number of adventitious buds per explant (16.71 +/- 0.34). adventitious buds elongated on dcr medium supplemented with 0.2 mg l(-1) ba and 0.02 mg l(-1) naa. after liquid pretreatment with 50 mg l(-1) indole-3-butyric acid (iba), over 95% of the shoots successfully rooted on 1/2 dcr medium supplemented with 0.3 mg l(-1) iba. the innovated systems reported in this study will be useful tools for future genetic manipulation of c. lanceolata and may be adapted for large-scale propagation in other conifers. |
| WOS关键词 | MATURE ZYGOTIC EMBRYOS ; CULTURES ; FRAGARIA ; L. |
| WOS研究方向 | Plant Sciences ; Cell Biology ; Developmental Biology |
| WOS类目 | Plant Sciences ; Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000251236000009 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382814 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wei, Zhi-Ming |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Mu-Lan,Wang, Ji,Yu, Yang,et al. Efficient organogenesis and plantlet regeneration in the timber species cunninghamia lanceolata (lamb.) hook[J]. In vitro cellular & developmental biology-plant,2007,43(5):449-455. |
| APA | Zhu, Mu-Lan,Wang, Ji,Yu, Yang,Liu, Sheng-Jun,&Wei, Zhi-Ming.(2007).Efficient organogenesis and plantlet regeneration in the timber species cunninghamia lanceolata (lamb.) hook.In vitro cellular & developmental biology-plant,43(5),449-455. |
| MLA | Zhu, Mu-Lan,et al."Efficient organogenesis and plantlet regeneration in the timber species cunninghamia lanceolata (lamb.) hook".In vitro cellular & developmental biology-plant 43.5(2007):449-455. |
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来源：中国科学院大学
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