The development of chromosome microdissection and microcloning technique and its applications in genomic research
文献类型：期刊论文
| 作者 | Zhou, Ruo-Nan; Hu, Zan-Min |
| 刊名 | Current genomics
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 8期号:1页码:67-72 |
| ISSN号 | 1389-2029 |
| 通讯作者 | Hu, zan-min(zmhu@genetics.ac.cn) |
| 英文摘要 | The technique of chromosome microdissection and microcloning has been developed for more than 20 years. as a bridge between cytogenetics and molecular genetics, it leads to a number of applications: chromosome painting probe isolation, genetic linkage map and physical map construction, and expressed sequence tags generation. during those 20 years, this technique has not only been benefited from other technological advances but also cross-fertilized with other techniques. today, it becomes a practicality with extensive uses. the purpose of this article is to review the development of this technique and its application in the field of genomic research. moreover, a new method of generating ests of specific chromosomes developed by our tab is introduced. by using this method, the technique of chromosome microdissection and microcloning would be more valuable in the advancement of genomic research. |
| WOS关键词 | WHEAT-THINOPYRUM-INTERMEDIUM ; RESISTANCE GENE ANALOGS ; POMELO CITRUS-GRANDIS ; RYE SECALE-CEREALE ; MOUSE X-CHROMOSOME ; WOODY FRUIT-TREES ; LASER MICRODISSECTION ; SINGLE CHROMOSOMES ; PCR AMPLIFICATION ; PAINTING PROBES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000244925700007 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382820 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Hu, Zan-Min |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ruo-Nan,Hu, Zan-Min. The development of chromosome microdissection and microcloning technique and its applications in genomic research[J]. Current genomics,2007,8(1):67-72. |
| APA | Zhou, Ruo-Nan,&Hu, Zan-Min.(2007).The development of chromosome microdissection and microcloning technique and its applications in genomic research.Current genomics,8(1),67-72. |
| MLA | Zhou, Ruo-Nan,et al."The development of chromosome microdissection and microcloning technique and its applications in genomic research".Current genomics 8.1(2007):67-72. |
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来源：中国科学院大学
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