Development of micropump-actuated negative pressure pinched injection for parallel electrophoresis on array microfluidic chip
文献类型：期刊论文
| 作者 | Li, Bowei1,2; Jiang, Lei1; Xie, Hua1,2; Gao, Yan1; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 30期号:17页码:3053-3057 |
| 关键词 | Array Injection Microfluidic chip Microvalve |
| 产权排序 | 1;1 |
| 通讯作者 | 秦建华 ; 林炳承 |
| 英文摘要 | a micropump-actuated negative pressure pinched injection method is developed for parallel electrophoresis on a multi-channel lif detection system. the system has a home-made device that could individually control 16-port solenoid valves and a high-voltage power supply. the laser beam is excitated and distributes to the array separation channels for detection. the hybrid glass-pdms microfluidic chip comprises two common reservoirs, four separation channels coupled to their respective pneumatic micropumps and two reference channels. due to use of pressure as a driving force, the proposed method has no sample bias effect for separation. there is only one high-voltage supply needed for separation without relying on the number of channels, which is significant for high-throughput analysis, and the time for sample loading is shortened to 1 s. in addition, the integrated micropumps can provide the versatile interface for coupling with other function units to satisfy the complicated demands. the performance is verified by separation of dna marker and hepatitis b virus dna samples. and this method is also expected to show the potential throughput for the dna analysis in the field of disease diagnosis. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | throughput genetic-analysis ; gated sample introduction ; capillary-electrophoresis ; microchip electrophoresis ; multichannel microchip ; performance ; devices ; system ; immunoassay ; separations |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000270015500012 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102071]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Bowei,Jiang, Lei,Xie, Hua,et al. Development of micropump-actuated negative pressure pinched injection for parallel electrophoresis on array microfluidic chip[J]. electrophoresis,2009,30(17):3053-3057. |
| APA | Li, Bowei,Jiang, Lei,Xie, Hua,Gao, Yan,Qin, Jianhua,&Lin, Bingcheng.(2009).Development of micropump-actuated negative pressure pinched injection for parallel electrophoresis on array microfluidic chip.electrophoresis,30(17),3053-3057. |
| MLA | Li, Bowei,et al."Development of micropump-actuated negative pressure pinched injection for parallel electrophoresis on array microfluidic chip".electrophoresis 30.17(2009):3053-3057. |
入库方式： OAI收割
来源：大连化学物理研究所
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