Long-term fertilization regimes affect bacterial community structure and diversity of an agricultural soil in northern china
文献类型：期刊论文
| 作者 | Ge, Yuan1,3; Zhang, Jia-bao2; Zhang, Li-mei1; Yang, Min1; He, Ji-zheng1 |
| 刊名 | Journal of soils and sediments
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 8期号:1页码:43-50 |
| 关键词 | 16s rrna gene clone library Bacterial diversity Denaturing gradient gel electrophoresis Inorganic fertilizers Long-term fertilization Organic manure |
| ISSN号 | 1439-0108 |
| DOI | 10.1065/jss2008.01.270 |
| 通讯作者 | He, ji-zheng(jzhe@rcees.ac.cn) |
| 英文摘要 | Background, aims, and scope. knowledge about shifts of microbial community structure and diversity following different agricultural management practices could improve our understanding of soil processes and thus help us to develop sound management strategies. a long-term fertilization experiment was established in 1989 at fengqiu (35 degrees 00'n, 114 degrees 24'e) in northern china. the soil (sandy loam) is classified as aquic inceptisols and has received continuous fertilization treatments since then. the fertilization treatments included control (ck, no fertilizer), chemical fertilizers nitrogen (n) and potassium (k) (nk), phosphorous (p) and k (pk), np, npk, organic manure (om), and half chemical fertilizers npk plus half organic manure (1/2npkom). the objective of this study was to examine if the microbial community structure and diversity were affected by the long-term fertilization regimes. materials and methods. soil samples were collected from the long-term experimental plots with seven treatments and four replications in april 2006. microbial dnas were extracted from the soil samples and the 16s rrna genes were pcr amplified. the pcr products were analyzed by dgge, cloning and sequencing. the bacterial community structures and diversity were assessed using the dgge profiles and the clone libraries constructed from the excised dgge bands. results. the bacterial community structure of the om and pk treatments were significantly different from those of all other treatments. the bacterial community structures of the four n-containing treatments (nk, np, npk and 1/2npkom), as well as ck, were more similar to each other. the changes in bacterial community structures of the om and pk treatments showed higher richness and diversity. phylogenetic analyses indicated that proteobacteria (30.5%) was the dominant taxonomic group of the soil, followed by acidobacteria (15.3%), gemmatimonadetes (12.7%), etc. discussion. irrespective of the two fertilization treatments of om and pk, the cluster analysis showed that bacterial communities of the remaining five treatments of ck, nk, np, npk and 1/2npkom seemed to be more similar to each other, which indicated the relatively weak effects of the four n-containing treatments on soil bacterial communities. n fertilizer may be considered as a key factor to counteract the effects of other fertilizers on microbial communities. conclusions. our results show that long-term fertilization regimes can affect bacterial community structure and diversity of the agricultural soil. the om and pk treatments showed a trend towards distinct community structures, higher richness and diversity when compared to the other treatments. contrasting to the positive effects of om and pk treatments on the bacterial communities, n fertilizer could be considered as a key factor in the soil to counteract the effects of other fertilizers on soil microbial communities. recommendations and perspectives. because of the extremely high abundance and diversity of microorganisms in soil and the high heterogeneity of the soil, it is necessary to further examine the effects of fertilization regimes on microbial community and diversity in different type soils for comprehensively understanding their effects through the appropriate combination of molecular approaches. |
| WOS关键词 | GRADIENT GEL-ELECTROPHORESIS ; RIBOSOMAL-RNA GENES ; MICROBIAL DIVERSITY ; SUBTROPICAL AUSTRALIA ; OXIDIZING BACTERIA ; SPECIES RICHNESS ; FOREST ; AGROECOSYSTEM ; BIODIVERSITY ; POPULATION |
| WOS研究方向 | Geology ; Agriculture |
| WOS类目 | Geosciences, Multidisciplinary ; Soil Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000253292000007 |
| 出版者 | SPRINGER HEIDELBERG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2384716 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, Ji-zheng |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Eco Environm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, State Expt Stn Agro Ecosyts Fengqiu, Nanjing 210008, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Yuan,Zhang, Jia-bao,Zhang, Li-mei,et al. Long-term fertilization regimes affect bacterial community structure and diversity of an agricultural soil in northern china[J]. Journal of soils and sediments,2008,8(1):43-50. |
| APA | Ge, Yuan,Zhang, Jia-bao,Zhang, Li-mei,Yang, Min,&He, Ji-zheng.(2008).Long-term fertilization regimes affect bacterial community structure and diversity of an agricultural soil in northern china.Journal of soils and sediments,8(1),43-50. |
| MLA | Ge, Yuan,et al."Long-term fertilization regimes affect bacterial community structure and diversity of an agricultural soil in northern china".Journal of soils and sediments 8.1(2008):43-50. |
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来源：中国科学院大学
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