Cytological characterization of the tandem repetitive sequences and their methylation status in the antirrhinum majus genome
文献类型：期刊论文
| 作者 | Zhang, Dongfen1,2,3; Yang, Qiuying3,4; Ding, Yong1,2,3; Cao, Xiaofeng1,2; Xue, Yongbiao4; Cheng, Zhukuan1,2 |
| 刊名 | Genomics
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 92期号:2页码:107-114 |
| 关键词 | Tandem repetitive sequences Fish Heterochromatin Methylation Antirrhinum |
| ISSN号 | 0888-7543 |
| DOI | 10.1016/j.ygeno.2008.04.008 |
| 通讯作者 | Cheng, zhukuan(zkcheng@genetics.ac.cn) |
| 英文摘要 | Tandem repetitive sequences are dna motifs common in the genomes of eukaryotic species and are often embedded in heterochromatic regions. in most eukaryotes, ribosomal genes, as well as centromeres and telomeres or subtelomeres, are associated with abundant tandem arrays of repetitive sequences and typically represent the final barriers to completion of whole-genome sequencing. the nature of these repeats makes it difficult to estimate their actual sizes. in this study, combining the two cytological techniques dna fiber-fish and pachytene chromosome fish allowed us to characterize the tandem repeats distributed genome wide in antirrhinum majus and identify four types of tandem repeats, 45s rdna, 5s rdna, centa1, and centa2, representing the major tandem repetitive components, which were estimated to have a total length of 18.50 mb and account for 3.59% of the a. majus genome. fish examination revealed that all the tandem repeats correspond to heterochromatic knobs along the pachytene chromosomes. moreover, the methylation status of the tandem repeats was investigated in both somatic cells and pollen mother cells from anther tissues using an antibody against 5-methylcytosine combined with sequential fish analyses. our results showed that these repeats were hypomethylated in anther tissues, especially in the pollen mother cells at pachytene stage. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | RIBOSOMAL-RNA GENES ; IN-SITU CHROMATIN ; DNA-METHYLATION ; ARABIDOPSIS-THALIANA ; ORYZA-SATIVA ; INTERGENIC SPACER ; DRAFT SEQUENCE ; DE-NOVO ; RICE ; CHROMOSOMES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000257981500005 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388026 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cheng, Zhukuan |
| 作者单位 | 1.State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Ctr Plant Gene Res, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, Mol & Dev Biol Lab, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dongfen,Yang, Qiuying,Ding, Yong,et al. Cytological characterization of the tandem repetitive sequences and their methylation status in the antirrhinum majus genome[J]. Genomics,2008,92(2):107-114. |
| APA | Zhang, Dongfen,Yang, Qiuying,Ding, Yong,Cao, Xiaofeng,Xue, Yongbiao,&Cheng, Zhukuan.(2008).Cytological characterization of the tandem repetitive sequences and their methylation status in the antirrhinum majus genome.Genomics,92(2),107-114. |
| MLA | Zhang, Dongfen,et al."Cytological characterization of the tandem repetitive sequences and their methylation status in the antirrhinum majus genome".Genomics 92.2(2008):107-114. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。