Contrasting effects of hc-pro and 2b viral suppressors from sugarcane mosaic virus and tomato aspermy cucumovirus on the accumulation of sirnas
文献类型：期刊论文
| 作者 | Zhang, Xiaoming1,2; Du, Peng1,2; Lu, Lu1,2; Xiao, Qi1,2; Wang, Wenjuan3; Cao, Xuesong1,2,4; Ren, Bo1,2; Wei, Chunhong1,2; Li, Yi1,2 |
| 刊名 | Virology
 |
| 出版日期 | 2008-05-10 |
| 卷号 | 374期号:2页码:351-360 |
| 关键词 | Rna silencing suppressor Sugarcane mosaic virus Hc-pro 2b 3' secondary sirna 5' secondary sirna Rdr6 |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2007.12.045 |
| 通讯作者 | Li, yi(liyi@pku.edu.cn) |
| 英文摘要 | Rna silencing and suppression of silencing are host and virus interactions for defense and counter-defense. here, we explored the function effect of hc-pro encoded by sugarcane mosaic virus (scmv) on the suppression of rna silencing. sirna northern blotting assay indicated that the replication of scmv was regulated by host rna silencing machinery. co-expression assay demonstrated that the hc-pro encoded by scmv suppressed the rna silencing induced by sense rna and dsrna. transitive rna silencing assay showed that hc-pro down-regulated the accumulation of 3' secondary sirna, but not 5' secondary and primary sirna. meanwhile, the 2b gene of tomato aspermy cucumovirus (tav) evidently down-regulated the accumulation of 5' secondary sirna. importantly, we found that hc-pro and tav2b down-regulated the accumulation of rdr6 mrna. thus, hc-pro, an rna silencing suppressor encoded by scmv, regulates the accumulation of different sirnas and has more than one target in the rna silencing pathway. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | DEPENDENT RNA-POLYMERASE ; DOUBLE-STRANDED-RNA ; TRANS-ACTING SIRNAS ; SILENCING SIGNAL ; INTERFERING RNA ; COAT PROTEIN ; CAENORHABDITIS-ELEGANS ; ANTIVIRAL IMMUNITY ; ENCODED SUPPRESSOR ; INITIATION STEP |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000255735900014 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2389126 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Yi |
| 作者单位 | 1.Peking Univ, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Life Sci, Natl Ctr Plant Gene Res Beijing, Beijing 100871, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China 4.Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaoming,Du, Peng,Lu, Lu,et al. Contrasting effects of hc-pro and 2b viral suppressors from sugarcane mosaic virus and tomato aspermy cucumovirus on the accumulation of sirnas[J]. Virology,2008,374(2):351-360. |
| APA | Zhang, Xiaoming.,Du, Peng.,Lu, Lu.,Xiao, Qi.,Wang, Wenjuan.,...&Li, Yi.(2008).Contrasting effects of hc-pro and 2b viral suppressors from sugarcane mosaic virus and tomato aspermy cucumovirus on the accumulation of sirnas.Virology,374(2),351-360. |
| MLA | Zhang, Xiaoming,et al."Contrasting effects of hc-pro and 2b viral suppressors from sugarcane mosaic virus and tomato aspermy cucumovirus on the accumulation of sirnas".Virology 374.2(2008):351-360. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。