Strain h(2)-419-4 of haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation
文献类型：期刊论文
| 作者 | Sun Yanhong1,2; Liu Jianguo1; Zhang Xiaoli1,2; Lin Wei1 |
| 刊名 | Chinese journal of oceanology and limnology
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 26期号:2页码:152-156 |
| 关键词 | Haematococcus pluvialis Astaxanthin Ethyl methanesulphonate (ems) Ultraviolet radiation (uv) |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-008-0152-y |
| 通讯作者 | Liu jianguo(jgliu@ms.qdio.ac.cn) |
| 英文摘要 | Two strains h(2)-410 and h(2)-419 were obtained from the chemically mutated survivors of wild haematococcus pluvialis 2 by using ethyl methanesulphonate (ems). strains h2-410 and h2-419 showed a fast cell growth with 13% and 20% increase in biomass compared to wild type, respectively. then h(2)-419-4, a fast cell growth and high astaxanthin accumulation strain, was obtained by exposing the strain h2-419 to ultraviolet radiation (uv) further. the total biomass, the astaxanthin content per cell, astaxanthin production of h(2)-4194 showed 68%, 28%, and 120% increase compared to wild h. pluvialis 2, respectively. hplc (high performance liquid chromatography) data showed also an obvious proportional variation of different carotenoid compositions in the extracts of h2-4194 and the wild type, although no peak of carotenoids appeared or disappeared. therefore, the main compositions in strain h(2)-419-4, like its wild one, were free of astaxanthin, monoester, and diester of astaxanthin. the asexual reproduction in survivors after exposed to uv was not synchronous, and different from the normal synchronous asexual reproduction as the mother cells were motile instead of non-motile. interestingly, some survivors from uv irradiation produced many mini-spores (or gamete?), the spores moved away from the mother cell gradually 4 or 5 days later. this is quite similar to sexual reproduction described by elliot in 1934. however, whether this was sexual reproduction remains questionable, as no mating process has been observed. |
| WOS关键词 | ASTAXANTHIN ; ACCUMULATION |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| WOS类目 | Limnology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000256416000005 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2389528 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu Jianguo |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun Yanhong,Liu Jianguo,Zhang Xiaoli,et al. Strain h(2)-419-4 of haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation[J]. Chinese journal of oceanology and limnology,2008,26(2):152-156. |
| APA | Sun Yanhong,Liu Jianguo,Zhang Xiaoli,&Lin Wei.(2008).Strain h(2)-419-4 of haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation.Chinese journal of oceanology and limnology,26(2),152-156. |
| MLA | Sun Yanhong,et al."Strain h(2)-419-4 of haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation".Chinese journal of oceanology and limnology 26.2(2008):152-156. |
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来源：中国科学院大学
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