中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Quantitative analysis of genistein in human plasma by online concentration ce with uv detection

文献类型:期刊论文

作者Feng, Hai-Yan1,2; Hou, Shu-Rong1; Zheng, Na1; Li, Xiang-Jun1; Hu, Zhong-Bo1; Yuan, Zhuo-Bin1
刊名Chromatographia
出版日期2008-09-01
卷号68期号:5-6页码:431-435
关键词Capillary electrophoresis Online acid barrage stacking Genistein Plasma
ISSN号0009-5893
DOI10.1365/s10337-008-0658-7
通讯作者Li, xiang-jun(lixiangj@gucas.ac.cn)
英文摘要A rapid capillary electrophoresis method based on online acid barrage stacking has been successfully established for analysis of trace amounts of genistein in human plasma. genistein was analyzed within 8 min, with 200 mmol l(-1) citric acid (ph 1.7) as acid barrage, and injection times of 180 and 30 s for sample and acid, respectively. good linearity was obtained in the range 0.05-5 mg l(-1) and the limit of detection was 0.03 mg l(-1). compared with normal sample injection, this method resulted in more than a fiftyfold improvement in detection sensitivity. the technique has potential for use in studies of genistein metabolism and of exposure levels in humans.
WOS关键词PERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; ISOFLAVONE AGLYCONES ; MASS-SPECTROMETRY ; DAIDZEIN
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
语种英语
WOS记录号WOS:000259186700019
出版者SPRINGER HEIDELBERG
URI标识http://www.irgrid.ac.cn/handle/1471x/2390973
专题中国科学院大学
通讯作者Li, Xiang-Jun
作者单位1.Chinese Acad Sci, Grad Univ, Coll Chem & Chem Engn, Beijing 100049, Peoples R China
2.Shijiazhuang Univ, Sch Chem Engn, Shijiazhuang 050035, Peoples R China
推荐引用方式
GB/T 7714
Feng, Hai-Yan,Hou, Shu-Rong,Zheng, Na,et al. Quantitative analysis of genistein in human plasma by online concentration ce with uv detection[J]. Chromatographia,2008,68(5-6):431-435.
APA Feng, Hai-Yan,Hou, Shu-Rong,Zheng, Na,Li, Xiang-Jun,Hu, Zhong-Bo,&Yuan, Zhuo-Bin.(2008).Quantitative analysis of genistein in human plasma by online concentration ce with uv detection.Chromatographia,68(5-6),431-435.
MLA Feng, Hai-Yan,et al."Quantitative analysis of genistein in human plasma by online concentration ce with uv detection".Chromatographia 68.5-6(2008):431-435.

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来源:中国科学院大学

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