A "nonsterile" method for selecting and growing arabidopsis thaliana transformants (t-2 transgenic lines) resistant to kanamycin
文献类型：期刊论文
| 作者 | Xu, Weifeng1,2; Shi, Weiming1 |
| 刊名 | Plant molecular biology reporter
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 26期号:4页码:350-357 |
| 关键词 | Arabidopsis thaliana Hydroponic system In solium selection Kanamycin Nonsterile conditions Seeding growth Select T-2 transgenic lines Transformants |
| ISSN号 | 0735-9640 |
| DOI | 10.1007/s11105-008-0052-5 |
| 通讯作者 | Shi, weiming(wmshi@issas.ac.cn) |
| 英文摘要 | A breakthrough in transgenic arabidopsis thaliana research was the development of the floral dip transformation protocol, a simple and reliable method of obtaining transformants, t-1 transgenic lines, at high efficiency while avoiding the use of tissue culture. however, the traditional protocol (a "sterile" method) of obtaining t-2 transgenic lines has not evolved along with improvements in transformation technology as it continues to be laborious and time-consuming. in this study, we report on the development of an improved protocol (a "nonsterile" method) for selecting and growing a. thaliana transformants (t-2 transgenic lines) resistant to kanamycin under nonsterile conditions. this protocol involves the use of a simple yet specialized device that will aid in solium selection of t-2 transgenic lines that can be rapidly grown in a hydroponic system. the "nonsterile" method reduces labor and time involved as compared to the "sterile" method; moreover, it is easy to set up and maintain. this method may also be applicable to other selecting agents, and perhaps to other plants. |
| WOS关键词 | HYDROPONIC METHOD ; LOW-MAINTENANCE ; SYSTEM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemical Research Methods ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000259962700008 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2391405 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shi, Weiming |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Weifeng,Shi, Weiming. A "nonsterile" method for selecting and growing arabidopsis thaliana transformants (t-2 transgenic lines) resistant to kanamycin[J]. Plant molecular biology reporter,2008,26(4):350-357. |
| APA | Xu, Weifeng,&Shi, Weiming.(2008).A "nonsterile" method for selecting and growing arabidopsis thaliana transformants (t-2 transgenic lines) resistant to kanamycin.Plant molecular biology reporter,26(4),350-357. |
| MLA | Xu, Weifeng,et al."A "nonsterile" method for selecting and growing arabidopsis thaliana transformants (t-2 transgenic lines) resistant to kanamycin".Plant molecular biology reporter 26.4(2008):350-357. |
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来源：中国科学院大学
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