Enhanced cell death by adcmv-p53 after irradiation of hela cells with c-12(6+) ions
文献类型：期刊论文
| 作者 | Liu, Bing1,2,3; Zhang, Hong1; Zhou, Guangming1; Xie, Yi1,2; Hao, Jifang1; Zhou, Qingming1,2; Duan, Xin1,2; Qiu, Rong1 |
| 刊名 | European journal of obstetrics gynecology and reproductive biology
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 138期号:2页码:226-231 |
| 关键词 | Carbon-ion beam P53 Cervix adenocarcinoma Adenovirus vector |
| ISSN号 | 0301-2115 |
| DOI | 10.1016/j.ejogrb.2007.08.012 |
| 通讯作者 | Liu, bing(liub626@126.com) |
| 英文摘要 | Objectives: to investigate whether the adenovirus-mediated p53 transfer could enhance the suppression of cervix adenocarcinoma by low-dose (<= 2.0 gy) heavy-ion irradiation. study design: hela cells were exposed to c-beam, and then infected with adcmv-p53 or gfp. p53 expression and cell cycle were detected by flow cytometric analysis. cell apoptosis was observed under a fluorescent microscope with dapi staining. the survival fraction was determined by colony forming assay. results: during the observation, the proportion of p53-positive cells in the c-beam with 80 moi p53 groups was significantly higher than that in control, c-beam only, 80 moi p53 only, and c-beam with 40 moi p53 (p < 0.05). the proportion of cells in g(1)- or g(2)-phase in the c-beam with p53 groups was significantly higher than that in the c-beam only groups, or p53 only groups (p < 0.05). the percentage of apoptotic cells for the c-beam with p53 was significantly higher than that for the c-beam only, or p53 only (p < 0.05). the survival fractions for the c-beam with p53 are significantly lower than those for the c-beam only, or p53 only (p < 0.05). sf2 for c-beam with 40 or 80 moi p53 decreased to similar to 10% and similar to 4%, respectively, compared with c-beam alone (similar to 42%). conclusions: adenovirus-mediated p53 transfer could enhance the suppression of cervix adenocarcinoma cells by low-dose c-beam irradiation. (c) 2007 elsevier ireland ltd. all rights reserved. |
| WOS关键词 | HUMAN-PAPILLOMAVIRUS TYPE-16 ; WILD-TYPE P53 ; GENE-TRANSFER ; LUNG-CANCER ; ADENOVIRUS ; GROWTH ; RADIOTHERAPY ; INFECTION |
| WOS研究方向 | Obstetrics & Gynecology ; Reproductive Biology |
| WOS类目 | Obstetrics & Gynecology ; Reproductive Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000257219100018 |
| 出版者 | ELSEVIER IRELAND LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2391703 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Bing |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Lanzhou Command Ctr Dis Prevent & Control, Lanzhou 730020, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Bing,Zhang, Hong,Zhou, Guangming,et al. Enhanced cell death by adcmv-p53 after irradiation of hela cells with c-12(6+) ions[J]. European journal of obstetrics gynecology and reproductive biology,2008,138(2):226-231. |
| APA | Liu, Bing.,Zhang, Hong.,Zhou, Guangming.,Xie, Yi.,Hao, Jifang.,...&Qiu, Rong.(2008).Enhanced cell death by adcmv-p53 after irradiation of hela cells with c-12(6+) ions.European journal of obstetrics gynecology and reproductive biology,138(2),226-231. |
| MLA | Liu, Bing,et al."Enhanced cell death by adcmv-p53 after irradiation of hela cells with c-12(6+) ions".European journal of obstetrics gynecology and reproductive biology 138.2(2008):226-231. |
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来源：中国科学院大学
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