The arabidopsis paraquat resistant2 gene encodes an s-nitrosoglutathione reductase that is a key regulator of cell death
文献类型：期刊论文
| 作者 | Chen, Ruiqiang1,2,3; Sun, Shulan1,2,3; Wang, Chun1,2,3; Li, Yansha1,2,3; Liang, Yan1,2; An, Fengying1,2,3; Li, Chao1,2,3; Dong, Haili1,2,3; Yang, Xiaohui1,2; Zhang, Jian1,2 |
| 刊名 | Cell research
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 19期号:12页码:1377-1387 |
| 关键词 | Gsnor1/hot5/par2 Nitric oxide Paraquat Cell death Superoxide |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2009.117 |
| 通讯作者 | Zuo, jianru(jrzuo@genetics.ac.cn) |
| 英文摘要 | Metabolism of s-nitrosoglutathione (gsno), a major biologically active nitric oxide (no) species, is catalyzed by the evolutionally conserved gsno reductase (gsnor). previous studies showed that the arabidopsis gsnor1/hot5 gene regulates salicylic acid signaling and thermotolerance by modulating the intracellular s-nitrosothiol level. here, we report the characterization of the arabidopsis paraquat resistant2-1 (par2-1) mutant that shows an anti-cell death phenotype. the production of superoxide in par2-1 is comparable to that of wild-type plants when treated by paraquat (1,1'-dimethyl-4,4'-bipyridinium dichloride), suggesting that par2 acts downstream of superoxide to regulate cell death. par2, identified by positional cloning, is shown to be identical to gsnor1/hot5. the par2-1 mutant carries a missense mutation in a highly conserved glycine, which renders the mutant protein unstable. compared to wild type, par2-1 mutant has a higher no level, as revealed by staining with 4,5-diaminofluorescein diacetate. consistent with this result, wild-type plants treated with an no donor display resistance to paraquat. interestingly, the gsnor1/hot5/par2 protein level, other than its steady-state mrna level, is induced by paraquat, but is reduced by no donors. taken together, these results suggest that gsnor1/hot5/par2 plays an important role in regulating cell death in plant cells through modulating intracellular no level. |
| WOS关键词 | DEPENDENT FORMALDEHYDE DEHYDROGENASE ; DISEASE RESISTANCE RESPONSE ; PLANT-PATHOGEN INTERACTIONS ; NITRIC-OXIDE ; ARABIDOPSIS-THALIANA ; SUPEROXIDE-DISMUTASE ; METHYL VIOLOGEN ; HYPERSENSITIVE RESPONSE ; NITROSYLATED PROTEINS ; ASCORBATE PEROXIDASE |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000273785100009 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2393905 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zuo, Jianru |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Plant Gene Res Ctr Beijing, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ruiqiang,Sun, Shulan,Wang, Chun,et al. The arabidopsis paraquat resistant2 gene encodes an s-nitrosoglutathione reductase that is a key regulator of cell death[J]. Cell research,2009,19(12):1377-1387. |
| APA | Chen, Ruiqiang.,Sun, Shulan.,Wang, Chun.,Li, Yansha.,Liang, Yan.,...&Zuo, Jianru.(2009).The arabidopsis paraquat resistant2 gene encodes an s-nitrosoglutathione reductase that is a key regulator of cell death.Cell research,19(12),1377-1387. |
| MLA | Chen, Ruiqiang,et al."The arabidopsis paraquat resistant2 gene encodes an s-nitrosoglutathione reductase that is a key regulator of cell death".Cell research 19.12(2009):1377-1387. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。