Stable non-synonymous substitutions on ns gene (ns1 and ns2 proteins) of qinghai lake h5n1 influenza virus (clade 2.2) after successive passages in muscovy ducks
文献类型：期刊论文
| 作者 | Song XiaoHui1,2,3; Huang Yu4; Xiao HaiXia1,2,3; Liu Di1,2; Gao, George F.1,2,3,5 |
| 刊名 | Science in china series c-life sciences
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 52期号:9页码:847-853 |
| 关键词 | Avian influenza virus H5n1 Mutation Ns1/2 Serial passages |
| ISSN号 | 1006-9305 |
| DOI | 10.1007/s11427-009-0115-3 |
| 通讯作者 | Gao, george f.(gaof@im.ac.cn) |
| 英文摘要 | Although worldwide concern has been raised since the large-scale outbreak of highly pathogenic avian influenza in wild birds at qinghai lake, china in 2005, the factors responsible for the ability to kill waterfowl remain unclear. the why and how questions of the h5n1 virus species-jump into its reservoir host need to be answered. in this report we test the pathogenicity and adaptation of qinghai lake (clade 2.2) isolate to muscovy ducks for further understanding of this virus. the isolate was highly pathogenic in ducks and retained its high pathogenicity even after 20 generations of passage in ducks. during the process of serial passages, only the ns gene developed non-synonymous substitutions, which caused two mutations in ns1 protein (val23ala and leu207pro) and one in ns2 (phe55leu). these mutations persisted immutably through all subsequent passages and the pathogenicity remained high, implying that highly pathogenic h5n1 virus remains stable in aquatic birds through oral transmission. although the exact functions of these mutations are not known, our results provide an important foundation for further understanding the characteristics of the qinghai lake isolates. |
| WOS关键词 | X-RAY-STRUCTURE ; AVIAN INFLUENZA ; MIGRATORY BIRDS ; A VIRUS ; CYGNUS-OLOR ; WILD BIRDS ; HONG-KONG ; EVOLUTION ; INFECTION ; GEESE |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| WOS类目 | Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000270489500009 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2397832 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, George F. |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol CAS, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Fujian Acad Agr Sci, Inst Anim Sci & Vet Med, Fuzhou 350013, Peoples R China 5.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song XiaoHui,Huang Yu,Xiao HaiXia,et al. Stable non-synonymous substitutions on ns gene (ns1 and ns2 proteins) of qinghai lake h5n1 influenza virus (clade 2.2) after successive passages in muscovy ducks[J]. Science in china series c-life sciences,2009,52(9):847-853. |
| APA | Song XiaoHui,Huang Yu,Xiao HaiXia,Liu Di,&Gao, George F..(2009).Stable non-synonymous substitutions on ns gene (ns1 and ns2 proteins) of qinghai lake h5n1 influenza virus (clade 2.2) after successive passages in muscovy ducks.Science in china series c-life sciences,52(9),847-853. |
| MLA | Song XiaoHui,et al."Stable non-synonymous substitutions on ns gene (ns1 and ns2 proteins) of qinghai lake h5n1 influenza virus (clade 2.2) after successive passages in muscovy ducks".Science in china series c-life sciences 52.9(2009):847-853. |
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来源：中国科学院大学
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