The thlaspi caerulescens nramp homologue tcnramp3 is capable of divalent cation transport
文献类型：期刊论文
| 作者 | Wei, Wei1; Chai, Tuanyao1; Zhang, Yuxiu2; Han, Lu1; Xu, Jin1; Guan, Ziqiu1 |
| 刊名 | Molecular biotechnology
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:1页码:15-21 |
| 关键词 | Heavy metal Metal transporter Nramp Thlaspi caerulescens Yeast |
| ISSN号 | 1073-6085 |
| DOI | 10.1007/s12033-008-9088-x |
| 通讯作者 | Chai, tuanyao(tychai@gucas.ac.cn) |
| 英文摘要 | The nramp gene family encodes integral membrane protein and mediates the transport of fe, however, its function in transport of toxic metal ions is not very clear in plants. tcnramp3 was isolated from thlaspi caerulescens, and encoded a metal transporter member of the nramp family. tcnramp3 was predominantly expressed in roots of t. caerulescens by semi-quantitative rt-pcr. the expression of tcnramp3 was induced by iron starvation and by the heavy metals cd and ni in roots. tcnramp3 was able to rescue growth of an iron uptake fet3fet4 mutant yeast strain, suggesting a possible role in iron transport. expression of tcnramp3 in yeast increased cd sensitivity and cd content, while it enhanced the ni resistance and reduced ni accumulation, indicating that tcnramp3 could accumulate cd and exclude ni in yeast. furthermore, overexpression of tcnramp3 in tobacco resulted in slight cd sensitivity of root growth and did not influence ni resistance. these results suggested that tcnramp3 played a role in metal cation homeostasis in plant. |
| WOS关键词 | METAL TRANSPORTER ; IRON TRANSPORT ; GENE ; ARABIDOPSIS ; RESISTANCE ; CLONING ; CANDIDATE ; ATNRAMP3 ; PROTEINS ; TOMATO |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000261692100003 |
| 出版者 | HUMANA PRESS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2401085 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Tuanyao |
| 作者单位 | 1.Grad Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.China Univ Min & Technol Beijing, Sch Chem & Environm Engn, Dept Bioengn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wei,Chai, Tuanyao,Zhang, Yuxiu,et al. The thlaspi caerulescens nramp homologue tcnramp3 is capable of divalent cation transport[J]. Molecular biotechnology,2009,41(1):15-21. |
| APA | Wei, Wei,Chai, Tuanyao,Zhang, Yuxiu,Han, Lu,Xu, Jin,&Guan, Ziqiu.(2009).The thlaspi caerulescens nramp homologue tcnramp3 is capable of divalent cation transport.Molecular biotechnology,41(1),15-21. |
| MLA | Wei, Wei,et al."The thlaspi caerulescens nramp homologue tcnramp3 is capable of divalent cation transport".Molecular biotechnology 41.1(2009):15-21. |
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来源：中国科学院大学
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