Phosphate release contributes to the rate-limiting step for unwinding by an rna helicase
文献类型：期刊论文
| 作者 | Wang, Qixin1,3; Arnold, Jamie J.3; Uchida, Akira3; Raney, Kevin D.2; Cameron, Craig E.3 |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 38期号:4页码:1312-1324 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkp1118 |
| 通讯作者 | Raney, kevin d.(raneykevind@uams.edu) |
| 英文摘要 | Rna helicases function in numerous aspects of rna biology. these enzymes are rna-stimulated atpases that translocate on rna and unwind or remodel structured rna in an atp-dependent fashion. how atp and the atpase cycle fuel the work performed by helicases is not completely clear. the hepatitis c virus rna helicase, ns3, is an important model system for this class of enzymes. ns3 binding to a single-/double-strand rna or dna junction leads to atp-independent melting of the duplex and formation of a complex capable of atp-dependent unwinding by using a spring-loaded mechanism. we have established an rna substrate for ns3 that can be unwound in a single sub-step. our studies are consistent with a model in which a single atp binding and/or hydrolysis event sets the unwinding spring and phosphate dissociation contributes to release of the spring, thereby driving the power stroke used for unwinding. |
| WOS关键词 | HEPATITIS-C VIRUS ; DEAD-BOX PROTEINS ; NS3 HELICASE ; ATP HYDROLYSIS ; STRAND SEPARATION ; BINDING-SITES ; IN-VITRO ; MECHANISM ; TRANSLOCATION ; REPLICATION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000275270500031 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2405920 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Raney, Kevin D. |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100049, Peoples R China 2.Univ Arkansas Med Sci, Dept Biochem & Mol Biol, Little Rock, AR 72205 USA 3.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA |
| 推荐引用方式 GB/T 7714 | Wang, Qixin,Arnold, Jamie J.,Uchida, Akira,et al. Phosphate release contributes to the rate-limiting step for unwinding by an rna helicase[J]. Nucleic acids research,2010,38(4):1312-1324. |
| APA | Wang, Qixin,Arnold, Jamie J.,Uchida, Akira,Raney, Kevin D.,&Cameron, Craig E..(2010).Phosphate release contributes to the rate-limiting step for unwinding by an rna helicase.Nucleic acids research,38(4),1312-1324. |
| MLA | Wang, Qixin,et al."Phosphate release contributes to the rate-limiting step for unwinding by an rna helicase".Nucleic acids research 38.4(2010):1312-1324. |
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来源：中国科学院大学
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