Srgap2 arginine methylation regulates cell migration and cell spreading through promoting dimerization
文献类型：期刊论文
| 作者 | Guo, Shaoshi2; Bao, Shilai1 |
| 刊名 | Journal of biological chemistry
 |
| 出版日期 | 2010-11-05 |
| 卷号 | 285期号:45页码:35133-35141 |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.m110.153429 |
| 通讯作者 | Bao, shilai(slbao@genetics.ac.cn) |
| 英文摘要 | The slit-robo gtpase-activating proteins (srgaps) are critical for neuronal migration through inactivation of rho gtpases cdc42, rac1, and rhoa. here we report that srgap2 physically interacts with protein arginine methyltransferase 5 (prmt5). srgap2 localizes to the cytoplasm and plasma membrane protrusion. srgap2 knockdown reduces cell adhesion spreading and increases cell migration, but has no effect on cell proliferation. prmt5 binds to the n terminus of srgap2 (225-538 aa) and methylates its c-terminal arginine residue arg-927. the methylation mutant srgap2-r927a fails to rescue the cell spreading rate, is unable to localize to the plasma membrane leading edge, and perturbs srgap2 homodimer formation mediated by the f-bar domain. these results suggest that srgap2 arginine methylation plays important roles in cell spreading and cell migration through influencing membrane protrusion. |
| WOS关键词 | F-BAR PROTEINS ; MEMBRANE INVAGINATION ; SIGNAL-TRANSDUCTION ; NEURONAL MIGRATION ; ACTIN CYTOSKELETON ; RHO-GTPASES ; DOMAIN ; PRMT5 ; METHYLTRANSFERASE ; METASTASIS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000283659100087 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2407165 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Bao, Shilai |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Mol & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Shaoshi,Bao, Shilai. Srgap2 arginine methylation regulates cell migration and cell spreading through promoting dimerization[J]. Journal of biological chemistry,2010,285(45):35133-35141. |
| APA | Guo, Shaoshi,&Bao, Shilai.(2010).Srgap2 arginine methylation regulates cell migration and cell spreading through promoting dimerization.Journal of biological chemistry,285(45),35133-35141. |
| MLA | Guo, Shaoshi,et al."Srgap2 arginine methylation regulates cell migration and cell spreading through promoting dimerization".Journal of biological chemistry 285.45(2010):35133-35141. |
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来源：中国科学院大学
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