A comparison between ribo-minus rna-sequencing and polya-selected rna-sequencing
文献类型：期刊论文
| 作者 | Cui, Peng1; Lin, Qiang1,2; Ding, Feng1; Xin, Chengqi1,2; Gong, Wei1,2; Zhang, Lingfang1,2; Geng, Jianing1; Zhang, Bing1; Yu, Xiaomin1; Yang, Jin1 |
| 刊名 | Genomics
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 96期号:5页码:259-265 |
| 关键词 | Ribominus Rna-seq Mrna-seq |
| ISSN号 | 0888-7543 |
| DOI | 10.1016/j.ygeno.2010.07.010 |
| 通讯作者 | Hu, songnian(husn@big.ac.cn) |
| 英文摘要 | To compare the two rna-sequencing protocols, ribo-minus rna-sequencing (rmrna-seq) and polya-selected rna-sequencing (mrna-seq), we acquired transcriptomic data-52 and 32 million alignable reads of 35 bases in length-from the mouse cerebrum, respectively. we found that a higher proportion, 44% and 25%, of the uniquely alignable rmrna-seq reads, is in intergenic and intronic regions, respectively, as compared to 23% and 15% from the mrna-seq dataset. further analysis made an additional discovery of transcripts of protein-coding genes (such as histone. heg1, and dux), ncrnas, snornas, snrnas, and novel ncrnas as well as repeat elements in rmrna-seq dataset. this result suggests that rmrna-seq method should detect more polya- or bimorphic transcripts. finally, through comparative analyses of gene expression profiles among multiple datasets, we demonstrated that different rna sample preparations may result in significant variations in gene expression profiles. (c) 2010 elsevier inc. all rights reserved. |
| WOS关键词 | CELL TRANSCRIPTOME ; HUMAN PROMOTERS ; MESSENGER-RNA ; REVEALS ; GENES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000283894300001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408250 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Hu, Songnian |
| 作者单位 | 1.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Peng,Lin, Qiang,Ding, Feng,et al. A comparison between ribo-minus rna-sequencing and polya-selected rna-sequencing[J]. Genomics,2010,96(5):259-265. |
| APA | Cui, Peng.,Lin, Qiang.,Ding, Feng.,Xin, Chengqi.,Gong, Wei.,...&Yu, Jun.(2010).A comparison between ribo-minus rna-sequencing and polya-selected rna-sequencing.Genomics,96(5),259-265. |
| MLA | Cui, Peng,et al."A comparison between ribo-minus rna-sequencing and polya-selected rna-sequencing".Genomics 96.5(2010):259-265. |
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来源：中国科学院大学
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