Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase ii detoxifying enzyme systems in retinal pigment epithelial cells
文献类型：期刊论文
| 作者 | Zhu, Lu2,3; Liu, Zhongbo2,3; Feng, Zhihui2,3; Hao, Jiejie2,3; Shen, Weili2; Li, Xuesen2,3; Sun, Lijuan4; Sharman, Edward5; Wang, Ying6; Wertz, Karin6 |
| 刊名 | Journal of nutritional biochemistry
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 21期号:11页码:1089-1098 |
| 关键词 | Hydroxytyrosol Rpe cells Acrolein Amd Mitochondrial biogenesis Phase ii enzymes |
| ISSN号 | 0955-2863 |
| DOI | 10.1016/j.jnutbio.2009.09.006 |
| 通讯作者 | Liu, jiankang(j.liu@mail.xjtu.edu.cn) |
| 英文摘要 | Studies in this laboratory have previously shown that hydroxytyrosol, the major antioxidant polyphenol in olives, protects arpe-19 human retinal pigment epithelial cells from oxidative damage induced by acrolein, an environmental toxin and endogenous end product of lipid oxidation, that occurs at increased levels in age-related macular degeneration lesions. a proposed mechanism for this is that protection by hydroxytyrosol against oxidative stress is conferred by the simultaneous activation of two critically important pathways, viz., induction of phase ii detoxifying enzymes and stimulation of mitochondrial biogenesis. cultured arpe-19 cells were pretreated with hydroxytyrosol and challenged with acrolein. the protective effects of hydroxytyrosol on key factors of mitochondrial biogenesis and phase ii detoxifying enzyme systems were examined. hydroxytyrosol treatment simultaneously protected against acrolein-induced inhibition of nuclear factor-e2-related factor 2 (nrf2) and peroxisome proliferator-activated receptor coactivator 1 alpha (ppargc1 alpha) in arpe-19 cells. the activation of nrf2 led to activation of phase ii detoxifying enzymes, including gamma-glutamyl-cysteinyl-ligase, nadph (nicotinamide adenine dinucleotide phosphate)-quinone-oxidoreductase 1, heme-oxygenase-1, superoxide dismutase, peroxiredoxin and thioredoxin as well as other antioxidant enzymes, while the activation of ppargc1 alpha led to increased protein expression of mitochondrial transcription factor a, uncoupling protein 2 and mitochondrial complexes. these results suggest that hydroxytyrosol is a potent inducer of phase ii detoxifying enzymes and an enhancer of mitochondrial biogenesis. dietary supplementation of hydroxytyrosol may contribute to eye health by preventing the degeneration of retinal pigment epithelial cells induced by oxidative stress. (c) 2010 elsevier inc. all rights reserved. |
| WOS关键词 | ALPHA-LIPOIC ACID ; MACULAR DEGENERATION ; OLIVE OIL ; GLUTATHIONE-PEROXIDASE ; CARDIOVASCULAR-DISEASE ; ANTIOXIDANT ACTIVITY ; MEDITERRANEAN DIET ; HYDROGEN-PEROXIDE ; DYSFUNCTION ; STRESS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Nutrition & Dietetics |
| WOS类目 | Biochemistry & Molecular Biology ; Nutrition & Dietetics |
| 语种 | 英语 |
| WOS记录号 | WOS:000283825600009 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408347 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Jiankang |
| 作者单位 | 1.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Inst Mitochondrial Biol & Med, Key Lab Biomed Informat Engn,Minist Educ, Xian 710049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.E China Normal Univ, Coll Phys Educ & Hlth, Shanghai 200062, Peoples R China 5.Univ Calif Irvine, Dept Neurol, Irvine, CA 92697 USA 6.DSM Nutr Prod R&D Human Nutr & Hlth, CH-4303 Basel, Switzerland 7.Univ Kentucky, Coll Med, Grad Ctr Toxicol, Lexington, KY 40536 USA |
| 推荐引用方式 GB/T 7714 | Zhu, Lu,Liu, Zhongbo,Feng, Zhihui,et al. Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase ii detoxifying enzyme systems in retinal pigment epithelial cells[J]. Journal of nutritional biochemistry,2010,21(11):1089-1098. |
| APA | Zhu, Lu.,Liu, Zhongbo.,Feng, Zhihui.,Hao, Jiejie.,Shen, Weili.,...&Liu, Jiankang.(2010).Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase ii detoxifying enzyme systems in retinal pigment epithelial cells.Journal of nutritional biochemistry,21(11),1089-1098. |
| MLA | Zhu, Lu,et al."Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase ii detoxifying enzyme systems in retinal pigment epithelial cells".Journal of nutritional biochemistry 21.11(2010):1089-1098. |
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来源：中国科学院大学
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