Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells
文献类型：期刊论文
| 作者 | Ding, Jie1,2,3,4; Wang, Yan4; Cheng, Tingting4; Chen, Xiaowei4; Gao, Bin1,2 |
| 刊名 | Immunological investigations
 |
| 出版日期 | 2010 |
| 卷号 | 39期号:2页码:103-113 |
| 关键词 | Hla-a24 Hla-a2 Single chain Mycobacterium tuberculosis Rma-s Binding Epitope |
| ISSN号 | 0882-0139 |
| 通讯作者 | Gao, bin(bgao2004@gmail.com) |
| 英文摘要 | Tuberculosis is caused by an intracellular pathogen mycobacterium tuberculosis (mtb) and poses a persistent threat to global health. mhc class i-restricted cd8 cytotoxic t lymphocytes (ctl) are essential for protective immunity to tuberculosis. information for ctl epitopes derived from mtb is desirable for vaccine design and assessment of t cell responses. however, the knowledge about ctl epitopes of mtb, particularly those non-a2 hla alleles restricted is rare. in this study, beta-2-microglobulin (beta 2m, beta(2)m) linked hla-a24 single chain was expressed on rma-s cell line defective in the endogenous antigen processing and applied for screening of peptides which could stabilize the hla-a24 complex on the cell surface. from a group of peptides predicted as binders by a computer algorithm, five peptides were shown to bind to hla-a24 protein on the cell surface. as comparison we have also identified a dozen mtb proteins derived peptides that bind to hla-a2 specifically. the cell line and hla binders present here would be useful for further identification of cd8 restricted mtb epitopes. |
| WOS关键词 | CLASS-I MOLECULES ; MAJOR HISTOCOMPATIBILITY COMPLEX ; SECONDARY ANCHOR RESIDUES ; CYTOTOXIC T-CELLS ; MONOCLONAL-ANTIBODIES ; BINDING ; INFECTION ; ANTIGENS ; ENUMERATION ; RESPONSES |
| WOS研究方向 | Immunology |
| WOS类目 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000274857100002 |
| 出版者 | TAYLOR & FRANCIS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408865 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, Bin |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol CASPMI, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Microbiol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Nanjing Ctr Dis Prevent & Control, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Jie,Wang, Yan,Cheng, Tingting,et al. Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells[J]. Immunological investigations,2010,39(2):103-113. |
| APA | Ding, Jie,Wang, Yan,Cheng, Tingting,Chen, Xiaowei,&Gao, Bin.(2010).Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells.Immunological investigations,39(2),103-113. |
| MLA | Ding, Jie,et al."Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells".Immunological investigations 39.2(2010):103-113. |
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来源：中国科学院大学
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