中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells

文献类型:期刊论文

作者Ding, Jie1,2,3,4; Wang, Yan4; Cheng, Tingting4; Chen, Xiaowei4; Gao, Bin1,2
刊名Immunological investigations
出版日期2010
卷号39期号:2页码:103-113
关键词Hla-a24 Hla-a2 Single chain Mycobacterium tuberculosis Rma-s Binding Epitope
ISSN号0882-0139
通讯作者Gao, bin(bgao2004@gmail.com)
英文摘要Tuberculosis is caused by an intracellular pathogen mycobacterium tuberculosis (mtb) and poses a persistent threat to global health. mhc class i-restricted cd8 cytotoxic t lymphocytes (ctl) are essential for protective immunity to tuberculosis. information for ctl epitopes derived from mtb is desirable for vaccine design and assessment of t cell responses. however, the knowledge about ctl epitopes of mtb, particularly those non-a2 hla alleles restricted is rare. in this study, beta-2-microglobulin (beta 2m, beta(2)m) linked hla-a24 single chain was expressed on rma-s cell line defective in the endogenous antigen processing and applied for screening of peptides which could stabilize the hla-a24 complex on the cell surface. from a group of peptides predicted as binders by a computer algorithm, five peptides were shown to bind to hla-a24 protein on the cell surface. as comparison we have also identified a dozen mtb proteins derived peptides that bind to hla-a2 specifically. the cell line and hla binders present here would be useful for further identification of cd8 restricted mtb epitopes.
WOS关键词CLASS-I MOLECULES ; MAJOR HISTOCOMPATIBILITY COMPLEX ; SECONDARY ANCHOR RESIDUES ; CYTOTOXIC T-CELLS ; MONOCLONAL-ANTIBODIES ; BINDING ; INFECTION ; ANTIGENS ; ENUMERATION ; RESPONSES
WOS研究方向Immunology
WOS类目Immunology
语种英语
WOS记录号WOS:000274857100002
出版者TAYLOR & FRANCIS INC
URI标识http://www.irgrid.ac.cn/handle/1471x/2408865
专题中国科学院大学
通讯作者Gao, Bin
作者单位1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol CASPMI, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Microbiol, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
4.Nanjing Ctr Dis Prevent & Control, Nanjing, Peoples R China
推荐引用方式
GB/T 7714
Ding, Jie,Wang, Yan,Cheng, Tingting,et al. Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells[J]. Immunological investigations,2010,39(2):103-113.
APA Ding, Jie,Wang, Yan,Cheng, Tingting,Chen, Xiaowei,&Gao, Bin.(2010).Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells.Immunological investigations,39(2),103-113.
MLA Ding, Jie,et al."Identification of hla-a24-binding peptides of mycobacterium tuberculosis derived proteins with beta 2m linked hla-a24 single chain expressing cells".Immunological investigations 39.2(2010):103-113.

入库方式: iSwitch采集

来源:中国科学院大学

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。