Comprehensive survey of human brain microrna by deep sequencing
文献类型：期刊论文
| 作者 | Shao, Ning-Yi2,3; Hu, Hai Yang2; Yan, Zheng2; Xu, Ying2; Hu, Hao1,2; Menzel, Corinna1; Li, Na4; Chen, Wei1,4; Khaitovich, Philipp2,5 |
| 刊名 | Bmc genomics
 |
| 出版日期 | 2010-06-30 |
| 卷号 | 11页码:14 |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/1471-2164-11-409 |
| 通讯作者 | Chen, wei(wei.chen@mdc-berlin.de) |
| 英文摘要 | Background: microrna (mirna) play an important role in gene expression regulation. at present, the number of annotated mirna continues to grow rapidly, in part due to advances of high-throughput sequencing techniques. here, we use deep sequencing to characterize a population of small rna expressed in human and rhesus macaques brain cortex. results: based on a total of more than 150 million sequence reads we identify 197 putative novel mirna, in humans and rhesus macaques, that are highly conserved among mammals. these putative mirna have significant excess of conserved target sites in genes' 3'utrs, supporting their functional role in gene regulation. additionally, in humans and rhesus macaques respectively, we identify 41 and 22 conserved putative mirna originating from non-coding rna (ncrna) transcripts. while some of these molecules might function as conventional mirna, others might be harmful and result in target avoidance. conclusions: here, we further extend the repertoire of conserved human and rhesus macaque mirna. even though our study is based on a single tissue, the coverage depth of our study allows identification of functional mirna present in brain tissue at background expression levels. therefore, our study might cover large proportion of the yet unannotated conserved mirna present in the human genome. |
| WOS关键词 | MESSENGER-RNAS ; BINDING-SITES ; GENE ; IDENTIFICATION ; EXPRESSION ; TARGETS ; GENOME ; CELLS ; TRANSCRIPTOME ; LIMITATIONS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000280399400001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2414590 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Wei |
| 作者单位 | 1.Max Planck Inst Mol Genet, D-14195 Berlin, Germany 2.Partner Inst Computat Biol, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, D-13092 Berlin, Germany 5.Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany |
| 推荐引用方式 GB/T 7714 | Shao, Ning-Yi,Hu, Hai Yang,Yan, Zheng,et al. Comprehensive survey of human brain microrna by deep sequencing[J]. Bmc genomics,2010,11:14. |
| APA | Shao, Ning-Yi.,Hu, Hai Yang.,Yan, Zheng.,Xu, Ying.,Hu, Hao.,...&Khaitovich, Philipp.(2010).Comprehensive survey of human brain microrna by deep sequencing.Bmc genomics,11,14. |
| MLA | Shao, Ning-Yi,et al."Comprehensive survey of human brain microrna by deep sequencing".Bmc genomics 11(2010):14. |
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来源：中国科学院大学
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