Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish
文献类型:期刊论文
作者 | Liu, Yanfang2; Guo, Zhimou2; Zhu, Jianhua1; Liang, Xinmiao2; Zhao, Fa1; Liu, Yanming1; Zhang, Xiqi1; Dong, Rui1; Yu, Wenjiang1 |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
出版日期 | 2018-10-26 |
卷号 | 1573页码:48-58 |
ISSN号 | 0021-9673 |
关键词 | Fish Arsenic species Protease Ammonium carbonate Kinetic energy discrimination HPLC-ICP-MS |
DOI | 10.1016/j.chroma.2018.08.068 |
通讯作者 | Liu, Yanming(msymliu@163.com) |
英文摘要 | A sensitive, simple and rapid method for the simultaneous determination of eleven arsenic species has been developed. A high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) technique was used for the analysis of eleven arsenic species in less than 17 min. Different extraction solutions were explored and the recovery results using water and aqueous acidic solvents, aqueous organic solvents and enzymes showed that 40 mg protease with 0.75 mL 0.5% hydrochloric acid (v/v) as the extraction agent gave the best experimental results. Species separation with ammonium carbonate solution as the mobile phase was conducted on an anion-exchange chromatographic column using gradient elution. The column temperature was 20 degrees C and kinetic energy discrimination (KED) was employed to eliminate spectral interference. The use of KED mode effectively removed interference from (ArCl)-Ar-75. The detection limit (L-D) of the method was in the range of 0.11-0.59 mu g kg(-1). Repeatability values obtained for spiked real fish samples were in the range of 1.1%-7.6%. Accuracy was calculated based on the analysis of spiked real fish samples at five concentration levels. Obtained recoveries were 91%-106%. The validated method was used in a pilot study to analyze real samples of fish, the organic arsenic especially AsB was the major arsenic specie present in the analyzed samples, only trace amount of inorganic arsenic were detected. The analytical method should improve the assessment of human exposure associated with arsenic intake from fish. (C) 2018 Elsevier B.V. All rights reserved. |
WOS关键词 | ATOMIC-ABSORPTION-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; HPLC-ICP-MS ; MULTIELEMENTAL SPECIATION ANALYSIS ; WATER SAMPLES ; FLUORESCENCE SPECTROMETRY ; DRINKING-WATER ; SEPARATION ; SEAFOOD ; SELENIUM |
资助项目 | National Key Research and Development Program of China[2017YFC1601600] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000447572400005 |
资助机构 | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166940] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Liu, Yanming |
作者单位 | 1.Shandong Inst Food & Drug Control, Jinan 250100, Shandong, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Yanfang,Guo, Zhimou,Zhu, Jianhua,et al. Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1573:48-58. |
APA | Liu, Yanfang.,Guo, Zhimou.,Zhu, Jianhua.,Liang, Xinmiao.,Zhao, Fa.,...&Yu, Wenjiang.(2018).Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish.JOURNAL OF CHROMATOGRAPHY A,1573,48-58. |
MLA | Liu, Yanfang,et al."Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish".JOURNAL OF CHROMATOGRAPHY A 1573(2018):48-58. |
入库方式: OAI收割
来源:大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。