中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish

文献类型:期刊论文

作者Liu, Yanfang2; Guo, Zhimou2; Zhu, Jianhua1; Liang, Xinmiao2; Zhao, Fa1; Liu, Yanming1; Zhang, Xiqi1; Dong, Rui1; Yu, Wenjiang1
刊名JOURNAL OF CHROMATOGRAPHY A
出版日期2018-10-26
卷号1573页码:48-58
ISSN号0021-9673
关键词Fish Arsenic species Protease Ammonium carbonate Kinetic energy discrimination HPLC-ICP-MS
DOI10.1016/j.chroma.2018.08.068
通讯作者Liu, Yanming(msymliu@163.com)
英文摘要A sensitive, simple and rapid method for the simultaneous determination of eleven arsenic species has been developed. A high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) technique was used for the analysis of eleven arsenic species in less than 17 min. Different extraction solutions were explored and the recovery results using water and aqueous acidic solvents, aqueous organic solvents and enzymes showed that 40 mg protease with 0.75 mL 0.5% hydrochloric acid (v/v) as the extraction agent gave the best experimental results. Species separation with ammonium carbonate solution as the mobile phase was conducted on an anion-exchange chromatographic column using gradient elution. The column temperature was 20 degrees C and kinetic energy discrimination (KED) was employed to eliminate spectral interference. The use of KED mode effectively removed interference from (ArCl)-Ar-75. The detection limit (L-D) of the method was in the range of 0.11-0.59 mu g kg(-1). Repeatability values obtained for spiked real fish samples were in the range of 1.1%-7.6%. Accuracy was calculated based on the analysis of spiked real fish samples at five concentration levels. Obtained recoveries were 91%-106%. The validated method was used in a pilot study to analyze real samples of fish, the organic arsenic especially AsB was the major arsenic specie present in the analyzed samples, only trace amount of inorganic arsenic were detected. The analytical method should improve the assessment of human exposure associated with arsenic intake from fish. (C) 2018 Elsevier B.V. All rights reserved.
WOS关键词ATOMIC-ABSORPTION-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; HPLC-ICP-MS ; MULTIELEMENTAL SPECIATION ANALYSIS ; WATER SAMPLES ; FLUORESCENCE SPECTROMETRY ; DRINKING-WATER ; SEPARATION ; SEAFOOD ; SELENIUM
资助项目National Key Research and Development Program of China[2017YFC1601600]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者ELSEVIER SCIENCE BV
WOS记录号WOS:000447572400005
资助机构National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China
源URL[http://cas-ir.dicp.ac.cn/handle/321008/166940]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Liu, Yanming
作者单位1.Shandong Inst Food & Drug Control, Jinan 250100, Shandong, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yanfang,Guo, Zhimou,Zhu, Jianhua,et al. Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1573:48-58.
APA Liu, Yanfang.,Guo, Zhimou.,Zhu, Jianhua.,Liang, Xinmiao.,Zhao, Fa.,...&Yu, Wenjiang.(2018).Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish.JOURNAL OF CHROMATOGRAPHY A,1573,48-58.
MLA Liu, Yanfang,et al."Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish".JOURNAL OF CHROMATOGRAPHY A 1573(2018):48-58.

入库方式: OAI收割

来源:大连化学物理研究所

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