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Light-sheet microscopy in the near-infrared II window

文献类型：期刊论文

作者Zhang, Mingxi7; Dai, Hongjie9; Liu LQ(刘连庆)3; Hong, Guosong1; Li WJ(李文荣)2,3; Liang, Yongye5; Luo, Jian4,6; Ma, Huilong5; Li, Lulin6; Du, Haotian9
刊名NATURE METHODS
出版日期2019
卷号16期号:6页码:545-552
ISSN号1548-7091
产权排序7
英文摘要Non-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000-1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 mu m through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site.
WOS关键词IN-VIVO ; FLUORESCENCE MICROSCOPY ; 2-PHOTON MICROSCOPY ; DEEP ; BRAIN ; TISSUE ; LASER ; DYE ; NANOPARTICLES ; WAVELENGTHS
资助项目National Institutes of Health[DP1-NS-105737]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000469455200024
资助机构National Institutes of Health
源URL[http://ir.sia.cn/handle/173321/24725]  
专题沈阳自动化研究所_机器人学研究室
通讯作者Dai, Hongjie
作者单位1.Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
2.Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
4.Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA
5.Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen, China
6.Palo Alto Veterans Institute for Research, VA Palo Alto Health Care System, Palo Alto, CA, USA
7.State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, China
8.Department of Radiology and BRIC, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
9.Department of Chemistry and Bio-X, Stanford University, Stanford, CA, USA
推荐引用方式
GB/T 7714		 Zhang, Mingxi,Dai, Hongjie,Liu LQ,et al. Light-sheet microscopy in the near-infrared II window[J]. NATURE METHODS,2019,16(6):545-552.
APA Zhang, Mingxi.,Dai, Hongjie.,Liu LQ.,Hong, Guosong.,Li WJ.,...&Wang FF.(2019).Light-sheet microscopy in the near-infrared II window.NATURE METHODS,16(6),545-552.
MLA Zhang, Mingxi,et al."Light-sheet microscopy in the near-infrared II window".NATURE METHODS 16.6(2019):545-552.

入库方式： OAI收割

来源：沈阳自动化研究所

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