Tracking motile algal cells with a deformable model
文献类型:期刊论文
作者 | Wen, Chenglu1; Wang, Pengbo1; Li, Wei1; Zhao, Jinquan2; Li, Rui1 |
刊名 | NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
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出版日期 | 2007-12-01 |
卷号 | 50期号:5页码:1285-1292 |
关键词 | Active Contour Algae Deformable Model Hough Transform Tracking |
ISSN号 | 0028-8233 |
英文摘要 | Phototaxis is a natural locomotor response of many mobile algal unicells either towards or away from a light stimulus. A novel, non-contact and non-invasive cell-tracking technique is described which can be used to monitor cell movernent. This is the first step towards developing an understanding of the organism's phototactic movement control system. A "defon-nable" modelling method has the potential to contribute significantly to tracking the motion of deformable cell types. We propose a segmenting and continuous tracking method based on a gradient vector flow (GVF) defori-nable model. To avoid the manual initialisation step of the earlier deformable models, a fast Hough transforrn method for detecting circles is used to obtain the whole contour of single and discrete cells and the watershed algorithm is used to obtain the initial contour of adjacent cells, even when in contact with one another. For the small capture range of the GVF edge energy, the deformable curves are easily bulged outward to the object boundary. This edge enhancing algorithm combined with the GVF defon-nable model could solve this problem. We present the tracking performance with real sequence images of Chlainydomonas reinhardtii cells under bright-field microscopy and indicate the validation of the method. |
语种 | 英语 |
WOS记录号 | WOS:000258308200094 |
出版者 | RSNZ PUBLISHING |
源URL | [http://ir.iccas.ac.cn/handle/121111/60497] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Li, Wei |
作者单位 | 1.China Agr Univ, Coll Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Key Lab Photochem, Inst Chem, Beijing 100080, Peoples R China |
推荐引用方式 GB/T 7714 | Wen, Chenglu,Wang, Pengbo,Li, Wei,et al. Tracking motile algal cells with a deformable model[J]. NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH,2007,50(5):1285-1292. |
APA | Wen, Chenglu,Wang, Pengbo,Li, Wei,Zhao, Jinquan,&Li, Rui.(2007).Tracking motile algal cells with a deformable model.NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH,50(5),1285-1292. |
MLA | Wen, Chenglu,et al."Tracking motile algal cells with a deformable model".NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH 50.5(2007):1285-1292. |
入库方式: OAI收割
来源:化学研究所
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