Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds
文献类型：期刊论文
| 作者 | Shen, Hong; Hu, Xixue; Yang, Fei; Bei, Jianzhong; Wang, Shenguo |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 32期号:13页码:3404-3412 |
| 关键词 | Bfgf Immobilization Slow Release Heparin Plga Scaffold |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2011.01.037 |
| 英文摘要 | In order to effectively and uniformly immobilize basic fibroblast growth factor (bFGF) to thick PLGA scaffold, the heparin-conjugated PLGA (H-PLGA) was synthesized at the first by reaction between heparin and a low molecular weight PLGA. Then heparin-containing PLGA (H-PLGA/PLGA) scaffold was fabricated by blending the H-PLGA with a high molecular weight PLGA. Finally, bFGF was immobilized on the H-PLGA/PLGA scaffold mainly by static electricity action between them. The effect of H-PLGA content on bFGF binding efficiency of the H-PLGA/PLGA scaffolds was investigated. It was found that bFGF binding efficiency increased with increasing H-PLGA content The bound bFGF can release in vitro slowly from the H-PLGA/PLGA scaffolds and last over two weeks. The released bFGF has still preserved its bioactivity. The attachment and growth of mouse 3T3 fibroblasts on the H-PLGA/PLGA scaffolds were better than that on the PLGA scaffold, however bFGF immobilized H-PLGA/PLGA scaffolds showed much better cell affinity. Therefore, the method to use the H-PLGA/PLGA scaffold for immobilizing bFGF is not only effective for slow delivering bFGF with bioactivity, but also can be used for fabricating thick scaffold where bFGF could be combined and uniformly distributed. (C) 2011 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000288884800007 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71555]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shenguo |
| 作者单位 | Chinese Acad Sci, Inst Chem, BNLMS, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Hong,Hu, Xixue,Yang, Fei,et al. Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds[J]. BIOMATERIALS,2011,32(13):3404-3412. |
| APA | Shen, Hong,Hu, Xixue,Yang, Fei,Bei, Jianzhong,&Wang, Shenguo.(2011).Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds.BIOMATERIALS,32(13),3404-3412. |
| MLA | Shen, Hong,et al."Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds".BIOMATERIALS 32.13(2011):3404-3412. |
入库方式： OAI收割
来源：化学研究所
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