Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus
文献类型：期刊论文
| 作者 | Yang, Yingli; Zhang, Feng; Zhao, Meigui; An, Lizhe; Zhang, Lixin; Chen, Nianlai |
| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 26期号:2页码:229-235 |
| 关键词 | H+-ATPase NaCl plasma membrane Populus euphratica callus thiol group |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-006-0220-8 |
| 通讯作者 | Yang, Yingli(yangyingli2006@sohu.com) |
| 英文摘要 | The plasma membrane (PM) vesicles from Populus euphratica (P. euphratica) callus were isolated to investigate the properties of the PM H+-ATPase. An enrichment of sealed and oriented right-side-out PM vesicles was demonstrated by measurement of the purity and orientation of membrane vesicles in the upper phase fraction. Analysis of pH optimum, temperature effects and kinetic properties showed that the properties of the PM H+-ATPase from woody plant P. euphratica callus were consistent with those from herbaceous species. Application of various thiol reagents to the reaction revealed that reduced thiol groups were essential to maintain the PM H+-ATPase activity. In addition, there was increased H+-ATPase activity in the PM vesicles when callus was exposed to NaCl. Western blotting analysis demonstrated an enhancement of H+-ATPase content in NaCl-treated P. euphratica callus compared with the control. |
| 收录类别 | SCI |
| WOS关键词 | HALOPHYTE ATRIPLEX-NUMMULARIA ; TRANSLOCATING ATPASES ; LIPID-COMPOSITION ; GENE-EXPRESSION ; PLANT-GROWTH ; PROTON PUMP ; STRESS ; NACL ; IDENTIFICATION ; TRANSPORT |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000243805500012 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555940 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Yang, Yingli |
| 作者单位 | 1.NW Normal Univ, Sch Life Sci, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yingli,Zhang, Feng,Zhao, Meigui,et al. Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus[J]. PLANT CELL REPORTS,2007,26(2):229-235. |
| APA | Yang, Yingli,Zhang, Feng,Zhao, Meigui,An, Lizhe,Zhang, Lixin,&Chen, Nianlai.(2007).Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus.PLANT CELL REPORTS,26(2),229-235. |
| MLA | Yang, Yingli,et al."Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus".PLANT CELL REPORTS 26.2(2007):229-235. |
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来源：寒区旱区环境与工程研究所
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