Bacterial community in the East Rongbuk Glacier, Mt. Qomolangma (Everest) by culture and culture-independent methods
文献类型：期刊论文
| 作者 | Zhang, Shu Hong2,3; Hou, Shu Gui1,3; Yang, Guang Li2; Wang, Jian Hui3 |
| 刊名 | MICROBIOLOGICAL RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 165期号:4页码:336-345 |
| 关键词 | Bacteria Abundance Community structure East Rongbuk Glacier Mt. Everest |
| ISSN号 | 0944-5013 |
| DOI | 10.1016/j.micres.2009.08.002 |
| 通讯作者 | Hou, Shu Gui(shugui@lzb.ac.cn) |
| 英文摘要 | The abundance of total and culturable bacteria deposited into the East Rongbuk ice core was investigated, and the bacterial content was examined through culture and culture-independent approaches. Total counts of bacteria in the ice core ranged from 0.02 x 10(3) to 6.4 x 10(3) cells ml(-1). Viable bacteria varied between 0 and 5.6 CFU ml(-1) on PYGV and R2A media. The phylogenetic trees placed the culturable bacteria into four major groups: Actinobacteria, Firmicutes, Proteobacteria and Deinococcus-Thermus, with Firmicutes being the most prevalent. They exhibited unique phenotypic properties with large and non-pigmented cells. The diversity revealed by H' index of DGGE analysis was 0-0.75, and closer sections showed higher similarity of bacterial DNA structure. Members of two major lineages were found: Firmicutes and Proteobacteria. The combined culture and culture-independent methods indicated layer distribution of bacterial community in the ice core section, which might reflect the ecological environments on glacier at time of their deposition. (c) 2009 Elsevier GmbH. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | ICE CORE ; ATMOSPHERIC CIRCULATION ; PHYSIOLOGICAL DIVERSITY ; MICROBIAL DIVERSITY ; SNOW ; CULTIVATION ; ENVIRONMENT ; HIMALAYA ; SITES ; FORMS |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000279002700009 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556285 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Hou, Shu Gui |
| 作者单位 | 1.Nanjing Univ, Sch Geog & Oceanog Sci, Nanjing 210093, Peoples R China 2.Shangqiu Normal Univ, Dept Life Sci, Shangqiu 476000, Peoples R China 3.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, State Key Lab Cryospher Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shu Hong,Hou, Shu Gui,Yang, Guang Li,et al. Bacterial community in the East Rongbuk Glacier, Mt. Qomolangma (Everest) by culture and culture-independent methods[J]. MICROBIOLOGICAL RESEARCH,2010,165(4):336-345. |
| APA | Zhang, Shu Hong,Hou, Shu Gui,Yang, Guang Li,&Wang, Jian Hui.(2010).Bacterial community in the East Rongbuk Glacier, Mt. Qomolangma (Everest) by culture and culture-independent methods.MICROBIOLOGICAL RESEARCH,165(4),336-345. |
| MLA | Zhang, Shu Hong,et al."Bacterial community in the East Rongbuk Glacier, Mt. Qomolangma (Everest) by culture and culture-independent methods".MICROBIOLOGICAL RESEARCH 165.4(2010):336-345. |
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来源：寒区旱区环境与工程研究所
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