Isocorydine induces cell differentiation through reversed epithelial mesenchymal transition in SiHa cells
文献类型：期刊论文
| 作者 | Liang, Xuefei1,2; Zeng, Jing1; Ding, Nan1; Liang, Zhongzhen1; Jian, Qiliang1; Du, Hong1; Liu, Junxi3; Wang, Fang1,2 |
| 刊名 | INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
 |
| 出版日期 | 2018 |
| 卷号 | 11期号:8页码:7811-+ |
| 关键词 | Isocorydine cell cycle arrest epithelial mesenchymal transition mesenchymal-epithelial transition antitumor SiHa cells |
| ISSN号 | 1940-5901 |
| 通讯作者 | Wang, Fang(fang4323@126.com) |
| 英文摘要 | Isocorydine (ICD) can be isolated from many plant materials and has been validated as a potential antitumor agent in some carcinomas. However, its role in cervical carcinoma remains unknown. Therefore, in this study, the mechanisms involved in isocorydine-induced suppression of cervical cancer were examined. MTT assay was used to examine the effects of ICD on proliferation of SiHa cells. Differential gene expression profiles in ICD-treated SiHa cells were analyzed with cDNA microarrays. Expression of HPV16E6, p53, NDRG1, CLCA2, and CASP14 in SiHa cells and tumor tissues was examined and Western blotting was used to analyze protein expression of epithelioid cell markers, such as E-cadherin and Vimentin. ICD reduced proliferation of SiHa cells, in vitro and in vivo. perhaps through p53-related pathways. Additionally, ICD regulated NDRG1, CLCA2, and CASP14 to suppress invasion and metastasis of SiHa cells and nude mice tumors. Histopathological examination of ICD-treated SiHa cells and nude mice tumors revealed morphologic changes suggesting differentiation, whereas Western blotting showed upregulated E-cadherin and downregulated vimentin. In conclusion, this study's findings reveal that ICD could suppress invasion and metastasis of SiHa cells by inducing G(1)/S cell cycle arrest and reversed epithelial mesenchymal transition. ICD might be a potential therapeutic for treatment of cervical cancer. |
| 收录类别 | SCI |
| WOS关键词 | HEPATOCELLULAR-CARCINOMA ; CENTRAL REGULATOR ; CANCER ; PLASTICITY ; APOPTOSIS ; MIGRATION ; TARGET ; CLCA2 |
| WOS研究方向 | Research & Experimental Medicine |
| WOS类目 | Medicine, Research & Experimental |
| 语种 | 英语 |
| WOS记录号 | WOS:000443533700030 |
| 出版者 | E-CENTURY PUBLISHING CORP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557764 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Wang, Fang |
| 作者单位 | 1.Lanzhou Univ, Hosp 2, Dept Reprod Med, Lanzhou, Gansu, Peoples R China 2.Lanzhou Univ, Hosp 2, Dept Obstet & Gynecol, Lanzhou, Gansu, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Xuefei,Zeng, Jing,Ding, Nan,et al. Isocorydine induces cell differentiation through reversed epithelial mesenchymal transition in SiHa cells[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2018,11(8):7811-+. |
| APA | Liang, Xuefei.,Zeng, Jing.,Ding, Nan.,Liang, Zhongzhen.,Jian, Qiliang.,...&Wang, Fang.(2018).Isocorydine induces cell differentiation through reversed epithelial mesenchymal transition in SiHa cells.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,11(8),7811-+. |
| MLA | Liang, Xuefei,et al."Isocorydine induces cell differentiation through reversed epithelial mesenchymal transition in SiHa cells".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 11.8(2018):7811-+. |
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来源：寒区旱区环境与工程研究所
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