Enhancement of radiosensitivity of MCF-7 breast cancer cells subjected to X-ray or carbon-ion irradiations
文献类型：期刊论文
| 作者 | Xie, Yi1,2,3 ; Wang, Bing4; Tanaka, Kaoru4; Li, Hong-Yan1,2,3; Zhao, Qiu-Yue1,2,3,5; Zhang, Hong1,2,3
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| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
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| 出版日期 | 2016-02-01 |
| 卷号 | 27页码:7 |
| 关键词 | Radiosensitivity Cellular cycle arrest BRCA1 P21 hTERT Telomere length |
| ISSN号 | 1001-8042 |
| DOI | 10.1007/s41365-016-0009-6 |
| 英文摘要 | The sensitivity of cancer cells to radiation therapy varies based on cell cycle phase. Here we evaluated the differences between X-ray and carbon-ion irradiation with respect to cellular radiosensitivity and cancer cycle arrest in the breast cancer cell line, MCF-7. The cell survival rate, cell cycle distribution and the presence of apoptosis were measured by clonogenic assay and flow cytometry. BRCA1 and p21 protein levels were analyzed by Western blot, and the levels of human telomerase reverse transcriptase (hTERT) mRNA expression and telomere length were detected with real-time polymerase chain reaction. The results show a significant dose-dependent effects on survival rate, apoptosis and protein levels in the carbon-ion group of MCF-7 cells. Decreased proliferation was not observed at 2 Gy X-ray irradiation. There were significant differences in cellular cycle arrest, apoptosis percentages and BRCA1 and p21 protein expression between X-ray and heavy-ion groups. The results indicated that increasing in BRCA1 and p21 expression, and attenuation of hTERT gene expression induced by heavy-ion irradiation in MCF-7 cells might relate to mechanism of cellular radiosensitivity in G(2)/M arrested phase. |
| WOS关键词 | TELOMERASE ACTIVITY ; CARCINOMA-CELLS ; BRCA1 ; PROLIFERATION ; GENES ; MOUSE ; CYCLE |
| 资助项目 | National Natural Science Foundation of China[U1432248] ; Gansu Provincial Science and Technology Department[0702NKDA045] ; Japan Society for the Promotion of Science ; National Institute of Radiological Sciences of Japan[11J364] |
| WOS研究方向 | Nuclear Science & Technology ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000376332800013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Natural Science Foundation of China ; Gansu Provincial Science and Technology Department ; Japan Society for the Promotion of Science ; National Institute of Radiological Sciences of Japan |
| 源URL | [http://119.78.100.186/handle/113462/42642]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou, Peoples R China 4.Natl Inst Radiol Sci, Chiba 260, Japan 5.Univ Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Yi,Wang, Bing,Tanaka, Kaoru,et al. Enhancement of radiosensitivity of MCF-7 breast cancer cells subjected to X-ray or carbon-ion irradiations[J]. NUCLEAR SCIENCE AND TECHNIQUES,2016,27:7. |
| APA | Xie, Yi,Wang, Bing,Tanaka, Kaoru,Li, Hong-Yan,Zhao, Qiu-Yue,&Zhang, Hong.(2016).Enhancement of radiosensitivity of MCF-7 breast cancer cells subjected to X-ray or carbon-ion irradiations.NUCLEAR SCIENCE AND TECHNIQUES,27,7. |
| MLA | Xie, Yi,et al."Enhancement of radiosensitivity of MCF-7 breast cancer cells subjected to X-ray or carbon-ion irradiations".NUCLEAR SCIENCE AND TECHNIQUES 27(2016):7. |
入库方式： OAI收割
来源：近代物理研究所
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