Effects of AdCMV-p53 gene transfer induced by irradiation on cycle of human colorectal adenocarcinoma cells
文献类型：期刊论文
| 作者 | Liu, Bing; Zhang, Hong ; Li, Wenjian; Gao, Qingxiang; Min, Fengling; Xie, Yi; Hao, Jifang; Duan, Xin; Zhou, Qingming; Zhou, Guangming
|
| 刊名 | Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing
 |
| 出版日期 | 2006 |
| 卷号 | 24页码:102-106 |
| ISSN号 | 1000-3436 |
| 英文摘要 | The work is to investigate effects of AdCMV-p53 gene transfer induced by 60Co γ-rays on cell cycles of human colorectal adenocarcinoma cells. HT-29 cells exposed to 0.5 Gy, 1.0 Gy and 2.0 Gy were infected with AdCMV-GFP, a replication deficient recombinant adenoviral vector containing a CMV promoter and green fluorescent protein, or AdCMV-p53, a replication deficient recombinant adenoviral vector containing a CMV promoter and carrying human wild-type p53 gene. Survival rate of the cells was determined by clonogenic assay. Cell cycle and cell apoptosis were determined by flow cytometry. The results showed that, 0.5-1.0 Gy irradiation significantly enhanced the inhibition of AdCMV-p53 infection on HT-29. Compared with the control, 1 day after the infection, the cells in G0/G10 phase decreased by 5%-15%, the cells in S phase increased by 2%-19%. The 0.5 and 1.0Gy irradiation made the cells in the in G2/M phase increase by 12%, infected with 80 MOI AdCMV-p5. Three days later, the proportion of cells in G2/M phase in groups of 0.5 Gyand 1.0 Gy irradiation + 40 MOI AdCMV-p53 infection increased by 10%-13%. There was a relation between cell apoptosis and irradiation dose, or AdCMV-p53 dose. Therefore, the irradiation-induction could quicken the progression from G0/G1 phase to S phase, and promote S and G2/M phase arrest. |
| 出版者 | Shanghai Science and Technology Press, Shanghai, China |
| 源URL | [http://119.78.100.186/handle/113462/64130]  |
| 专题 | 中国科学院近代物理研究所 |
| 作者单位 | 1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Graduate School, Chinese Academy of Sciences, Beijing 100039, China 3.School of Life Science, Lanzhou University, Lanzhou 730000, China |
| 推荐引用方式 GB/T 7714 | Liu, Bing,Zhang, Hong,Li, Wenjian,et al. Effects of AdCMV-p53 gene transfer induced by irradiation on cycle of human colorectal adenocarcinoma cells[J]. Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing,2006,24:102-106. |
| APA | Liu, Bing.,Zhang, Hong.,Li, Wenjian.,Gao, Qingxiang.,Min, Fengling.,...&Zhou, Guangming.(2006).Effects of AdCMV-p53 gene transfer induced by irradiation on cycle of human colorectal adenocarcinoma cells.Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing,24,102-106. |
| MLA | Liu, Bing,et al."Effects of AdCMV-p53 gene transfer induced by irradiation on cycle of human colorectal adenocarcinoma cells".Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing 24(2006):102-106. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。