Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation
文献类型：期刊论文
| 作者 | Liu, Bing; Zhang, Hong ; Li, Wenjian; Gao, Qingxiang; Min, Fengling; Xie, Yi; Zhou, Qingming; Duan, Xin; Zhou, Guangming
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| 刊名 | He Jishu/Nuclear Techniques
 |
| 出版日期 | 2006 |
| 卷号 | 29页码:674-678 |
| ISSN号 | 0253-3219 |
| 英文摘要 | The effect of AdCMV-p53 gene transfection induced by y-ray irradiation on human colorectal adenocarcinoma cells was investigated. The HT-29 cells were irradiated by 0.5, 1.0, 2.0 Gy 60Co y-rays, then were transfected with AdCMV-GFP (a replication of deficient recombinant adenoviral vector containing a CMV promoter and green fluorescent protein) or AdCMV-p53 (a replication of deficient recombinant adenoviral vector containing a CMV promoter and carrying human wild p53 gene). Cytotoxity was measured by clonogenic survival assay; apoptosis and the p53 expression were determined by flow cytometry. The results show that the pre-exposure of 0.5 Gy 60Co y-rays significantly enhanced the inhibition of HT-29 cells with AdCMV-p53 transfection and promoted cell apoptosis. The inhibition rates for the groups of pre-exposure with 0.5 Gy and transfection with 40 and 80 MOI AdCMV-p53 were 50% and 20% higher than those for the groups of the mere transfection, and 40% more than the mere irradiation group. In the case of higher than 0.5 Gy pre-exposure, no significant difference was found between the pre-exposure with transfection group and the mere irradiation group. So 0.5 Gy pre-irradiation and AdCMV-p53 transfection obviously increases the inhibition of HT-29 cells with AdCMV-p53 transfection. The optimum condition is the lower than 1.0 Gy pre-exposure combined with the lower than 80 MOI AdCMV-p53 transfection. |
| 出版者 | Science Press, Beijing, China |
| 源URL | [http://119.78.100.186/handle/113462/64140]  |
| 专题 | 中国科学院近代物理研究所 |
| 作者单位 | 1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Graduate School, Chinese Academy of Sciences, Beijing 100049, China 3.School of Life Science, Lanzhou University, Lanzhou 730000, China |
| 推荐引用方式 GB/T 7714 | Liu, Bing,Zhang, Hong,Li, Wenjian,et al. Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation[J]. He Jishu/Nuclear Techniques,2006,29:674-678. |
| APA | Liu, Bing.,Zhang, Hong.,Li, Wenjian.,Gao, Qingxiang.,Min, Fengling.,...&Zhou, Guangming.(2006).Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation.He Jishu/Nuclear Techniques,29,674-678. |
| MLA | Liu, Bing,et al."Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation".He Jishu/Nuclear Techniques 29(2006):674-678. |
入库方式： OAI收割
来源：近代物理研究所
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