Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function
文献类型：期刊论文
| 作者 | Liu, Bing1,2; Zhang, Hong1 ; Duan, Xin1; Hao, Pang1; Xie, Yi1; Zhou, Qingming1; Wang, Yanling1; Tian, Yuan2; Wang, Tao2
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| 刊名 | CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
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| 出版日期 | 2009-02-01 |
| 卷号 | 24期号:1页码:57-65 |
| 关键词 | carbon-ion beam subclinical-dose p53 gene transfer non-small-cell lung cancer adenovirus vector radiosensitivity |
| ISSN号 | 1084-9785 |
| DOI | 10.1089/cbr.2008.0514 |
| 英文摘要 | To determine whether adenovirus-mediated wild-type p53 transfer after radiotherapy Could radiosensitize non-small-cell lung cancer (NSCLC) cells to subclinical-dose carbon-ion beam (C-beam), H1299 cells were exposed to a C-beam or gamma-ray and then infected with 5 MOI of AdCMV-p53 or GFP (C-beam or gamma-ray with p53 or GFP). Cell cycle was detected by flow cytometric analysis. The apoptosis was examined by a fluorescent microscope with DAPI staining. DNA fragmentation was monitored by the TUNEL assay. P53 mRNA was detected by reverse-transcriptase polymerase chain reaction. The expression of p53, MDM(2), and p21 was monitored by Western blot. Survival fractions were determined by colony-forming assay. The percentages of G(1)-phase cells in C-beam with p53 increased by 8.2%-16.0%, 5.2%-7.0%, and 5.8%-18.9%, respectively, compared with C-beam only, gamma-ray with p53, or p53 only. The accumulation of G(2)-phase cells in C-beam with p53 increased by 5.7%-8.9% and 8.8%-14.8%, compared with those in gamma-ray with p53 or p53 only, respectively. The percentage of apoptosis for C-beam with p53 increased by 7.4%-19.1%, 5.8%-11.7%, and 5.2%-19.2%, respectively, compared with C-beam only, gamma-ray with p53, or p53 only. The level of p53 mRNA in C-beam with p53 was significantly higher than that in p53 only. The expression level of p53 and p21 in C-beam with p53 was significantly higher than that in both C-beam with GFP and p53 only. The survival fractions for C-beam with p53 were significantly less than those for the other groups (p < 0.05). The data suggested that AdCMV-p53 transfer could more efficiently radiosensitize H1299 cells to subclinical-dose C-beam irradiation through the restoration of p53 function. |
| WOS关键词 | RADIATION-INDUCED APOPTOSIS ; IONIZING-RADIATION ; GENE-TRANSFER ; GLIOMA-CELLS ; RADIOTHERAPY ; THERAPY ; CARCINOMA ; CANCER ; SUPPRESSION ; LINES |
| 资助项目 | National Natural Science Foundation of China[10675151] ; Key Scientific Technology Research Project of Gansu Province[2GS052-A43-008-02] ; Nature Science Foundation of Gansu Province[3ZS061-A25-021] |
| WOS研究方向 | Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000263898300009 |
| 出版者 | MARY ANN LIEBERT INC |
| 资助机构 | National Natural Science Foundation of China ; Key Scientific Technology Research Project of Gansu Province ; Nature Science Foundation of Gansu Province |
| 公开日期 | 2009-11-20 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/170]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 73000, Peoples R China 2.Lanzhou Command Ctr Dis Control & Prevent, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Bing,Zhang, Hong,Duan, Xin,et al. Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2009,24(1):57-65. |
| APA | Liu, Bing.,Zhang, Hong.,Duan, Xin.,Hao, Pang.,Xie, Yi.,...&Wang, Tao.(2009).Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,24(1),57-65. |
| MLA | Liu, Bing,et al."Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 24.1(2009):57-65. |
入库方式： OAI收割
来源：近代物理研究所
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