Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation
文献类型：期刊论文
| 作者 | Liu, Bing; Zhang, Hong; Zhou, Guangming; Xie, Yi; Hao, Jifang; Qiu, Rong; Duan, Xin; Zhou, Qingming |
| 刊名 | JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 42期号:2页码:140-145 |
| 关键词 | carbon-ion beam p53 hepatocellular carcinoma gene transfer adenovirus vector |
| ISSN号 | 0944-1174 |
| DOI | 10.1007/s00535-006-1977-9 |
| 英文摘要 | Background. The purpose of this study was to investigate whether adenovirus-mediated p53 transfer could sensitize hepatocellular carcinoma to heavy-ion irradiation. Methods. HepG2 cells were preexposed to a C-12(6+) beam, and then infected with replication-deficient adenovirus recombinant vectors containing human wild-type p53 (AdCMV-p53) (C-12(6+) irradiation + AdCMV-p53 infection). The survival fraction was determined by clonogenic assay. The cell cycle, cell apoptosis, and p53 expression were monitored by flow cytometric analysis. Results. p53 expression in C-12(6+) irradiation + AdCMV-p53 infection groups was markedly higher than that in C-12(6+) irradiation only groups (P < 0.05), suggesting that the preexposure to the C-12(6+) beam promoted the expression of exogenous p53 in HepG2 cells infected with AdCMV-p53 only. The G(1)-phase arrest and cell apoptosis in the C-12(6+) irradiation + AdCMV-p53 infection groups were significantly more than those in the C-12(6+) irradiated groups (P < 0.05). The survival fractions of the C-12(6+) irradiation + AdCMV-p53 infection groups decreased by 30%-49% compared with those of the C-12(6+) beam-irradiated only groups (P < 0.05). Conclusions. Adenovirus-mediated p53 gene transfer can promote G(1)-phase arrest and cell apoptosis, thus sensitizing hepatocellular carcinoma cells to heavy-ion irradiation. |
| WOS关键词 | TUMOR-SUPPRESSOR GENE ; IONIZING-RADIATION ; CYCLE CONTROL ; MUTANT P53 ; CANCER ; THERAPY ; RADIOTHERAPY ; EFFICACY ; RADIOSENSITIVITY ; MUTATIONS |
| WOS研究方向 | Gastroenterology & Hepatology |
| 语种 | 英语 |
| WOS记录号 | WOS:000244836100005 |
| 出版者 | SPRINGER TOKYO |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/6033]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Liu, Bing |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Bing,Zhang, Hong,Zhou, Guangming,et al. Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation[J]. JOURNAL OF GASTROENTEROLOGY,2007,42(2):140-145. |
| APA | Liu, Bing.,Zhang, Hong.,Zhou, Guangming.,Xie, Yi.,Hao, Jifang.,...&Zhou, Qingming.(2007).Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation.JOURNAL OF GASTROENTEROLOGY,42(2),140-145. |
| MLA | Liu, Bing,et al."Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation".JOURNAL OF GASTROENTEROLOGY 42.2(2007):140-145. |
入库方式： OAI收割
来源：近代物理研究所
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