High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation
文献类型：期刊论文
| 作者 | Duan, Xin1,3; Liu, Bing1,2,3; Zhang, Hong1 ; Zhou, Qingming1,3; Luo, Xiaohong2; Xie, Yi1,3; Hao, Jifang1
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| 刊名 | EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
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| 出版日期 | 2008-09-01 |
| 卷号 | 20期号:9页码:860-864 |
| 关键词 | adenovirus vector carbon-ion beam gene therapy irradiation p53 |
| ISSN号 | 0954-691X |
| DOI | 10.1097/MEG.0b013e3282f945e8 |
| 英文摘要 | Objective To investigate whether the irradiation with C-beam could enhance adenovirus-mediated transfer and expression of p53 in human hepatocellular carcinoma. Materials and methods HepG2 cells were exposed to C-beam or gamma-ray and then infected with replicationdeficient adenovirus recombinant vectors containing human wild-type p53 or green fluorescent protein, respectively. The transfer efficiency and expression level of the exogenous gene were detected by flow cytometric analysis. Cell survival fraction was detected by clonogenic assay. Results The transfer frequency in C-beam or gamma-irradiated groups increased by 50-83% and 5.7-38.0% compared with the control, respectively (P < 0.05). Compared with C-beam alone, p53 alone, and gamma-ray with p53, the percentages of p53 positive cells for 1 Gy C-beam with p53 increased by 56.0-72.0%, 63.5-82.0%, and 31.3-72.5% on first and third day after the treatments, respectively (P < 0.05). The survival fractions for the 2Gy C-bearn and AdCMV-p53 infection groups decreased to similar to 2%. Conclusion C-beam irradiation could significantly promote AdCMV-green fluorescent protein transfer and expression of p53. |
| WOS关键词 | WILD-TYPE P53 ; HUMAN-GENE-THERAPY ; IONIZING-RADIATION ; MEMBRANE-PERMEABILITY ; MAMMALIAN-CELLS ; GLIOMA-CELLS ; ADENOVIRUS ; RADIOTHERAPY ; REPORTER ; CANCER |
| 资助项目 | National Natural Science Foundation of China[10675151] ; Key Scientific Technology Research Project of Gansu Province[2GS052-A43-008-02] ; Nature Science Foundation of Gansu Province[3ZS061-A25-021] |
| WOS研究方向 | Gastroenterology & Hepatology |
| 语种 | 英语 |
| WOS记录号 | WOS:000259344200008 |
| 出版者 | LIPPINCOTT WILLIAMS & WILKINS |
| 资助机构 | National Natural Science Foundation of China ; Key Scientific Technology Research Project of Gansu Province ; Nature Science Foundation of Gansu Province |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/6323]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China 2.Lanzhou Command Ctr Dis Prevent & Control, Lanzhou, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Xin,Liu, Bing,Zhang, Hong,et al. High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation[J]. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY,2008,20(9):860-864. |
| APA | Duan, Xin.,Liu, Bing.,Zhang, Hong.,Zhou, Qingming.,Luo, Xiaohong.,...&Hao, Jifang.(2008).High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation.EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY,20(9),860-864. |
| MLA | Duan, Xin,et al."High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation".EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY 20.9(2008):860-864. |
入库方式： OAI收割
来源：近代物理研究所
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