Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes
文献类型：期刊论文
| 作者 | Chen, Yingtai3; Zhang, Yawei7; Liu, Hongliang5; Zhu, Zijiang5; Zhang, Xiaowei1; Ren, Jinyu6; Chen, Xuezhong5; Xie, Yi4 ; Zhang, Hong4; Li, Yumin2
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| 刊名 | ADVANCES IN SPACE RESEARCH
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| 出版日期 | 2010-04-01 |
| 卷号 | 45期号:7页码:832-838 |
| 关键词 | C-12(6+) ion X-ray Human Lymphocyte Spare |
| ISSN号 | 0273-1177 |
| DOI | 10.1016/j.asr.2009.09.024 |
| 英文摘要 | The aim of this study was to estimate the acute effects of low dose C-12(6+) ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05 Gy C-12(6+) ions or X-ray radiation and cell responses were measured at 24 h after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supematant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-gamma and TNF-alpha in HPBL and their protein levels in supernatant were significantly increased at 24 h after exposure to 0.05 Gy C-12(6+) ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05 Gy high linear energy transfer (LET) C-12(6+) radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDL (C) 2009 COSPAR. Published by Elsevier Ltd. All rights reserved. |
| WOS关键词 | TOTAL-BODY IRRADIATION ; EXTRATHYMIC T-CELLS ; IONIZING-RADIATION ; ADAPTIVE RESPONSE ; INTERFERON-GAMMA ; MICE ; INTERLEUKIN-2 ; GROWTH ; MELANOMA ; HORMESIS |
| 资助项目 | National Science Foundation of China[30770639] ; National Science Foundation of China[30870364] ; National Institute of Health (NIH)[ID43TW008323-01] ; National Institute of Health (NIH)[ID43TW007864-01] |
| WOS研究方向 | Astronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000276179300002 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Science Foundation of China ; National Institute of Health (NIH) |
| 公开日期 | 2011-04-20 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/8001]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Li, Yumin |
| 作者单位 | 1.Lanzhou Univ, Cent Lab, Hosp 1, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou 730030, Gansu, Peoples R China 3.Lanzhou Univ, Coll Clin Med, Lanzhou 730000, Gansu, Peoples R China 4.Chinese Acad Sci, Dept Radiol, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 5.Inst Med Sci Gansu Prov, Lanzhou 730050, Gansu, Peoples R China 6.Univ Kansas, Kidney Inst, Med Ctr, Kansas City, KS 66160 USA 7.Yale Univ, Sch Publ Hlth, New Haven, CT 06520 USA |
| 推荐引用方式 GB/T 7714 | Chen, Yingtai,Zhang, Yawei,Liu, Hongliang,et al. Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes[J]. ADVANCES IN SPACE RESEARCH,2010,45(7):832-838. |
| APA | Chen, Yingtai.,Zhang, Yawei.,Liu, Hongliang.,Zhu, Zijiang.,Zhang, Xiaowei.,...&Li, Yumin.(2010).Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes.ADVANCES IN SPACE RESEARCH,45(7),832-838. |
| MLA | Chen, Yingtai,et al."Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes".ADVANCES IN SPACE RESEARCH 45.7(2010):832-838. |
入库方式： OAI收割
来源：近代物理研究所
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