Determination of Bovine Insulin Solution Concentration Using Atomic Force Microscopy
文献类型：会议论文
| 作者 | Zhan ZK(詹志坤); Steve Tung; T. J. Mitchell; Dong ZL(董再励) ; Li WJ(李文荣)
|
| 出版日期 | 2008 |
| 会议名称 | The 2nd IEEE International Conference on Nano/Molecular Medicine and Engineering(IEEE-NanoMed) |
| 会议日期 | November 6-9, 2008 |
| 会议地点 | Suzhou, China, 2008 |
| 关键词 | insulin crystallization Atomic Force Microscopy concentration determination |
| 页码 | 4 pp. |
| 中文摘要 | A novel method based on Atomic Force Microscopy (AFM) and digital light microscopy (DLM) to measure the concentration of bovine insulin solution is presented. To overcome the limited sensitivities of the conventional methods of protein trace detection (such as Lowry method, Ultraviolet Absorption Method, Coomassie Bright Blue Dye-binding Method), a new approach of determining insulin concentration by calculating the volume of insulin crystals on a mica substrate using the softwares of AFM and DLM is developed. In this approach, sulfuric sodium was used as a precipitant reagent, and hydrochloric acid and sodium hydroxide were used to adjust the sample pH value to the insulin isoelectric point of ~5.35. Insulin crystals were formed by a simple evaporation method at the room temperature. The resultant crystal growth is observed and analyzed by the AFM and DLM. The up-to-date experimental results show that the combination of AFM and DLM is a feasible method to determine the concentration of insulin and other protein solutions, although the error margins must be improved in order for the method to become practical for clinical usage. |
| 产权排序 | 1 |
| 会议主办者 | IEEE |
| 会议录 | The 2nd IEEE International Conference on Nano/Molecular Medicine and Engineering(IEEE-NanoMed)
 |
| 会议录出版者 | IEEE |
| 会议录出版地 | New York |
| 语种 | 英语 |
| 源URL | [http://ir.sia.cn/handle/173321/8646]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Zhan ZK,Steve Tung,T. J. Mitchell,et al. Determination of Bovine Insulin Solution Concentration Using Atomic Force Microscopy[C]. 见:The 2nd IEEE International Conference on Nano/Molecular Medicine and Engineering(IEEE-NanoMed). Suzhou, China, 2008. November 6-9, 2008. |
入库方式： OAI收割
来源：沈阳自动化研究所
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