Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip
文献类型：期刊论文
| 作者 | Chau, LH; Liang WF(梁文峰); Cheung, FWK; Liu, WK; Li WJ(李文荣) ; Chen, SC; Lee, GB
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:1页码:1-8 |
| 关键词 | ELECTRIC-FIELD DIELECTROPHORESIS MANIPULATION SEPARATION |
| ISSN号 | 1932-6203 |
| 产权排序 | 2 |
| 通讯作者 | Liu, WK ; 李文荣 |
| 中文摘要 | The use of optical dielectrophoresis (ODEP) to manipulate microparticles and biological cells has become increasingly popular due to its tremendous flexibility in providing reconfigurable electrode patterns and flow channels. ODEP enables the parallel and free manipulation of small particles on a photoconductive surface on which light is projected, thus eliminating the need for complex electrode design and fabrication processes. In this paper, we demonstrate that mouse cells comprising melan-a cells, RAW 267.4 macrophage cells, peripheral white blood cells and lymphocytes, can be manipulated in an opto-electrokinetics (OEK) device with appropriate DEP parameters. Our OEK device generates a non-rotating electric field and exerts a localized DEP force on optical electrodes. Hitherto, we are the first group to report that among all the cells investigated, melan-a cells, lymphocytes and white blood cells were found to undergo self-rotation in the device in the presence of a DEP force. The rotational speed of the cells depended on the voltage and frequency applied and the cells' distance from the optical center. We discuss a possible mechanism for explaining this new observation of induced self-rotation based on the physical properties of cells. We believe that this rotation phenomenon can be used to identify cell type and to elucidate the dielectric and physical properties of cells. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ELECTRIC-FIELD ; DIELECTROPHORESIS ; MANIPULATION ; SEPARATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313429800009 |
| 公开日期 | 2013-04-21 |
| 源URL | [http://ir.sia.cn/handle/173321/10636]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Chau, LH,Liang WF,Cheung, FWK,et al. Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip[J]. PLOS ONE,2013,8(1):1-8. |
| APA | Chau, LH.,Liang WF.,Cheung, FWK.,Liu, WK.,Li WJ.,...&Lee, GB.(2013).Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip.PLOS ONE,8(1),1-8. |
| MLA | Chau, LH,et al."Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip".PLOS ONE 8.1(2013):1-8. |
入库方式： OAI收割
来源：沈阳自动化研究所
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