Measurement of interchain binding affinity of nucleic acid duplex using atomic force microscopy
文献类型：会议论文
| 作者 | Zhang, Tianbiao; Zhang CL(张常麟); Dong ZL(董再励) ; Guan, Yifu
|
| 出版日期 | 2010 |
| 会议名称 | 4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010 |
| 会议日期 | December 5-9, 2010 |
| 会议地点 | Hong Kong/Macau, China |
| 关键词 | Atomic force microscopy Binding energy Cluster analysis DNA Experiments Hydrogen Precision engineering Sodium Sodium chloride |
| 页码 | 125-129 |
| 中文摘要 | Atomic force microscope (AFM), as its high resolution and precision, was employed to directly probe intermolecular hydrogen bonds between self-assembled purines and pyrimidines. In the experiment, we directly stretch the DNA double strands that are attached to the planar modified glass surfaces and AFM tip respectively. However, distinguishing from previous literatures we focus on the NaCl density and holding time influencing the rupture force of DNA double helix and monitor the probing process under three different densities of sodium chloride (NaCl): 0mM, 50mM, and 100mM. The result shows complementary DNA strands are easier to bond as the density of NaCl increases. At the same time we test the probing process considering three holding durations: 0S, 10S, and 40S. We find the complementary DNA strands bond forming well under the situation of 40S. Then we separately do experiments with three lengths of strands: 10bp, 14bp, 20bp. Using cluster analysis method we find that the clustering center intervals are about 0.21nN, 0.29nN, and 0.39nN, and we can calculate the hydrogen bond of the single G-C is about 20pN. |
| 收录类别 | EI |
| 产权排序 | 2 |
| 会议主办者 | IEEE Nanotechnology Council; Chinese International NEMS Society; City University of Hong Kong; The Chinese University of Hong Kong; The University of Hong Kong; University of Macau |
| 会议录 | 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
 |
| 会议录出版者 | IEEE Computer Society |
| 会议录出版地 | Piscataway, USA |
| 语种 | 英语 |
| ISBN号 | 978-1-61284-153-3 |
| 源URL | [http://ir.sia.cn/handle/173321/10470]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Zhang, Tianbiao,Zhang CL,Dong ZL,et al. Measurement of interchain binding affinity of nucleic acid duplex using atomic force microscopy[C]. 见:4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010. Hong Kong/Macau, China. December 5-9, 2010. |
入库方式： OAI收割
来源：沈阳自动化研究所
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