中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)

文献类型：期刊论文

作者Huang, Baoyu1,2,3,5; Tang, Xueying1,2,3,5; Zhang, Linlin1,3,5; Li, Li1,2,4,5; Wang, Wei1,2,4,5; Liu, Mingkun1,2,5; Zhang, Guofan1,2,3,5
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-10-01
卷号93页码:551-558
关键词Oyster Innate immunity IKKe TBK1 NF-kappa B IRF
ISSN号1050-4648
DOI10.1016/j.fsi.2019.07.074
通讯作者Li, Li(lili@qdio.ac.cn) ; Zhang, Guofan(gzhang@qdio.ac.cn)
英文摘要IxB-related kinase epsilon (IKK epsilon) plays a crucial role in the activation of nuclear factor kappa B (NF-kappa B) by phosphorylating inhibitor of NF-kappa B (I kappa B) and in the regulation of interferon (IFN) gene expression by phosphorylating IFN regulatory factors (IRFs). In this study, we cloned an IKK epsilon homologue cDNA (designated as CgIKK epsilon-like) from the Pacific oyster, Crassostrea gigas. The full 2896-bp cDNA sequence comprised a 2163-bp open reading frame (ORF) encoding 720 amino acids. CgIKK epsilon-like is ubiquitously expressed, and its mRNA levels in hemocytes after poly I:C, V. alginolyticus, or OsHV-1 mu Var challenge were analyzed by real-time PCR. Compared to that in the control, CgIKK epsilon-like mRNA expression levels were significantly increased at 3 h and peaked at 6 h after OsHV-1 mu Var challenge; no obvious changes were observed in expression levels until 24 h after either V. alginolyticus or poly I:C challenge, reaching a maximum at 24 h (p < 0.01) and then rapidly decreasing. CgIKK epsilon-like transfection into human cell lines induced NF-kappa B and ISRE activation, while transfection with CgIKK epsilon-like deletion mutants abolished NF-kappa B and ISRE reporter gene activation. Additionally, CgIKK epsilon-like could interact with CgTBK1 and could form homodimers strongly, which may be critical for the immune signaling transduction. Last but not least, we found that CgIKK epsilon-like may increase CgI kappa Bs phosphorylation and could interact with CgIRF8. Together, these results suggest that CgIKK epsilon-like could respond to pathogenic infection, participate in the immune signal transduction and activate NF-kappa B and ISRE reporter genes. Thus, CgIKK epsilon-like could play an important role in the oyster immune system.
资助项目National Natural Science Foundation of China[31802328] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science[MS2018NO02] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000488998300061
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
源URL[http://ir.qdio.ac.cn/handle/337002/163250]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Li; Zhang, Guofan
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Baoyu,Tang, Xueying,Zhang, Linlin,et al. IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:551-558.
APA Huang, Baoyu.,Tang, Xueying.,Zhang, Linlin.,Li, Li.,Wang, Wei.,...&Zhang, Guofan.(2019).IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas).FISH & SHELLFISH IMMUNOLOGY,93,551-558.
MLA Huang, Baoyu,et al."IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)".FISH & SHELLFISH IMMUNOLOGY 93(2019):551-558.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。