The nitrate reductase gene-switch: A system for regulated expression in transformed cells of Dunaliella salina
文献类型：期刊论文
| 作者 | Li, He; Xue, Lexun; Yana, Hongxia; Wang, Lill; Liu, Lingling; Lu, Yumin; Xie, Hua
|
| 刊名 | GENE
 |
| 出版日期 | 2007-11-15 |
| 卷号 | 403期号:1-2页码:132-142 |
| 关键词 | bar gene Dunaliella salina expression nitrate reductase promoter |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2007.08.001 |
| 文献子类 | Article |
| 英文摘要 | The control of promoter activity by nitrogen source has recently emerged as an intriguing system for regulated expression of the heterologous genes. The purpose of this work was to investigate whether heterologous gene expression in transgenic Dunaliella salina would be controlled by an inducible promoter. Here we identify that the nitrate reductase (NR) transcripts of D. salina are induced by nitrate but repressed by ammonium. The bar gene integrated into the genome of D. salina is transcribed by a promoter of the NR gene from D. salina and the bar transcripts are induced by nitrate but repressed by ammonium. PPT-resistance of transformants disappears when they are transferred from nitrate-containing medium to ammonium-containing medium. The findings of this study demonstrate that the promoter of the D. salina NR gene can be used to control expression of the heterologous genes in transgenic D. salina. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | TRANSCRIPTIONAL REGULATION ; CHLAMYDOMONAS-REINHARDTII ; FUNCTIONAL-ANALYSIS ; PROMOTER ; CLONING ; SEQUENCES ; PLANTS |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000250610300015 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273112]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xue, Lexun |
| 作者单位 | 1.Zhengzhou Univ, Affiliated Hosp 1, Cell Biol Lab, Zhengzhou 450052, Henan, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, He,Xue, Lexun,Yana, Hongxia,et al. The nitrate reductase gene-switch: A system for regulated expression in transformed cells of Dunaliella salina[J]. GENE,2007,403(1-2):132-142. |
| APA | Li, He.,Xue, Lexun.,Yana, Hongxia.,Wang, Lill.,Liu, Lingling.,...&Xie, Hua.(2007).The nitrate reductase gene-switch: A system for regulated expression in transformed cells of Dunaliella salina.GENE,403(1-2),132-142. |
| MLA | Li, He,et al."The nitrate reductase gene-switch: A system for regulated expression in transformed cells of Dunaliella salina".GENE 403.1-2(2007):132-142. |
入库方式： OAI收割
来源：上海药物研究所
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