A new assay based on fluorescence resonance energy transfer to determine the binding affinity of BCl-x(L) inhibitors
文献类型：期刊论文
| 作者 | Feng, Yu1; Shen, Xu1,3 ; Chen, Kaixian2; Jiang, Hualiang2,3; Liu, Dongxiang1
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| 刊名 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2008-07 |
| 卷号 | 72期号:7页码:1936-1939 |
| 关键词 | fluorescence resonance energy transfer Bcl-x(L) inhibitor binding assay apoptosis |
| ISSN号 | 0916-8451 |
| DOI | 10.1271/bbb.70735 |
| 文献子类 | Article |
| 英文摘要 | We developed a new assay of BCl-x(L) inhibitors based on fluorescence resonance energy transfer that occurs between an AEDANS-labeled Bak-BH3 peptide and three tryptophans in the BH1 and BH2 domains of Bcl-x(L). The method can tolerate up to 5% DMSO, and it was validated with several BCl-x(L) inhibitors. It can be adapted to screen for compounds targeting other Bcl-2 family proteins. |
| WOS关键词 | BCL-2 FAMILY PROTEINS ; PEPTIDE COMPLEX ; TUMOR-CELLS ; APOPTOSIS ; REGULATORS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000258124700039 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272871]  |
| 专题 | 药理学第三研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药物发现与设计中心 |
| 通讯作者 | Liu, Dongxiang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Mol Pharmacol, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Ctr Drug Design & Discovery, Shanghai 201203, Peoples R China; 3.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Yu,Shen, Xu,Chen, Kaixian,et al. A new assay based on fluorescence resonance energy transfer to determine the binding affinity of BCl-x(L) inhibitors[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2008,72(7):1936-1939. |
| APA | Feng, Yu,Shen, Xu,Chen, Kaixian,Jiang, Hualiang,&Liu, Dongxiang.(2008).A new assay based on fluorescence resonance energy transfer to determine the binding affinity of BCl-x(L) inhibitors.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,72(7),1936-1939. |
| MLA | Feng, Yu,et al."A new assay based on fluorescence resonance energy transfer to determine the binding affinity of BCl-x(L) inhibitors".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 72.7(2008):1936-1939. |
入库方式： OAI收割
来源：上海药物研究所
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