Bcl-x(L) forms two distinct homodimers at non-ionic detergents: Implications in the dimerization of Bcl-2 family proteins
文献类型：期刊论文
| 作者 | Feng, Yu2; Lin, Zhaohu1; Shen, Xu1,3 ; Chen, Kaixian3; Jiang, Hualiang1,3; Liu, Dongxiang2
|
| 刊名 | JOURNAL OF BIOCHEMISTRY
 |
| 出版日期 | 2008-02 |
| 卷号 | 143期号:2页码:243-252 |
| 关键词 | apoptosis Bcl-2 family protein dimerization domain swapping non-ionic detergent |
| ISSN号 | 0021-924X |
| DOI | 10.1093/jb/mvm216 |
| 文献子类 | Article |
| 英文摘要 | As the key regulator of apoptosis, Bcl-2 family protein controls the cell death by forming homo- or heterodimers among anti-apoptotic and pro-apoptotic members of this family. Here we have studied Bcl-x(L) homodimerization at different pH in the presence of various detergents and organic solvents. We found that both acidic and basic pHs are beneficial for Bcl-x(L) dimerization. High concentrations of non-ionic detergents and some organic solvents can significantly promote this event. In addition to non-covalently linked acidic-dimer as that formed at acidic pH, Bcl-x(L) formed disulphide-bonded detergent-dimer at neutral and basic pH when incubated with high concentrations of non-ionic detergents. The acidic-dimer retains the BH3 peptide binding activity, whereas the detergent-dimer does not. The formation of acidic-dimer and detergent-dimer implies that Bcl-x(L) may dimerize via two different pathways under certain conditions. The implications of these findings has been discussed with previous experimental results, which provides some new insight into the events and would help the experiment design and data interpretation when non-ionic detergents are used to study the dimerization and pore formation of Bcl-2 family proteins. |
| WOS关键词 | PROGRAMMED CELL-DEATH ; PEPTIDE COMPLEX ; BAX OLIGOMERIZATION ; MONOMERIC PROTEIN ; X-L ; APOPTOSIS ; MITOCHONDRIA ; HETERODIMERIZATION ; CONFORMATION ; REGULATORS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000254840500012 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272995]  |
| 专题 | 药理学第三研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药物发现与设计中心 |
| 通讯作者 | Liu, Dongxiang |
| 作者单位 | 1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Mol Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Ctr Drug Design & Discovery, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Yu,Lin, Zhaohu,Shen, Xu,et al. Bcl-x(L) forms two distinct homodimers at non-ionic detergents: Implications in the dimerization of Bcl-2 family proteins[J]. JOURNAL OF BIOCHEMISTRY,2008,143(2):243-252. |
| APA | Feng, Yu,Lin, Zhaohu,Shen, Xu,Chen, Kaixian,Jiang, Hualiang,&Liu, Dongxiang.(2008).Bcl-x(L) forms two distinct homodimers at non-ionic detergents: Implications in the dimerization of Bcl-2 family proteins.JOURNAL OF BIOCHEMISTRY,143(2),243-252. |
| MLA | Feng, Yu,et al."Bcl-x(L) forms two distinct homodimers at non-ionic detergents: Implications in the dimerization of Bcl-2 family proteins".JOURNAL OF BIOCHEMISTRY 143.2(2008):243-252. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。