Caspase-8 preferentially senses the apoptosis-inducing action of NG-18, a gambogic acid derivative, in human leukemia HL-60 cells
文献类型：期刊论文
| 作者 | Tao, Zhijian; Zhou, Yunlong; Lu, Jinjian; Duan, Wenhu ; Qin, Yuxin; He, Xinxia; Lin, Liping; Ding, Jian
|
| 刊名 | CANCER BIOLOGY & THERAPY
 |
| 出版日期 | 2007-05 |
| 卷号 | 6期号:5页码:691-696 |
| 关键词 | gambogic acid NG-18 HL-60 apoptosis caspase-8 Bcl-2 anti-tumor |
| ISSN号 | 1538-4047 |
| DOI | 10.4161/cbt.6.5.3960 |
| 文献子类 | Article |
| 英文摘要 | Gambogic acid (GA) is the major active ingredient of gamboge secreted from a Chinese traditional medicine Garcinia hanburryi possessing potent anti-tumor activity. N-(2-ethoxyethyl)gambogamide (NG-18), a derivative of GA, also efficiently inhibits proliferation of cultured human tumor cells. The inhibition effect of NG-18 is associated with its ability to induce apoptosis. In the present study, NG-18 markedly induced leukemia HL-60 cells apoptosis, and the extrinsic and intrinsic apoptosis pathways were activated almost at the same time. NG-18-induced tumor cell apoptosis was associated with up-regulation of pro-apoptotic Bcl-2 family member Bax, and downregulation of anti-apoptotic protein Bcl-2. The NG-18-induced apoptosis was blocked completely by a pan-caspase inhibitor Z-VAD-FMK, indicating that caspases were functionally and actively involved in this process. The specific inhibition of caspase-8 activity using Z-IETD-FMK significantly blocked NG-18-induced apoptosis. In contrast, inhibition of other initiator caspases, caspase-2 or -9, using Z-VDVAD-FMK or Z-LEHD-FMK respectively had no effect on NG-18-induced apoptosis. Altogether, our data demonstrated that NG-18-induced apoptosis was dependent on caspases and caspase-8 acted as a key executor in the event. |
| WOS关键词 | DNA FRAGMENTATION ; CYTOCHROME-C ; IN-VITRO ; ACTIVATION ; MITOCHONDRIA ; PROLIFERATION ; DOWNSTREAM ; ETOPOSIDE ; RECEPTOR ; PATHWAY |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000248259900022 |
| 出版者 | LANDES BIOSCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273267]  |
| 专题 | 药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药理学第一研究室 |
| 通讯作者 | Ding, Jian |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Synth Med Chem, Shanghai 200031, Peoples R China 2.Zhejiang Normal Univ, Coll Chem & Life, Jinhua Zhejiang, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Anti Tumor Pharmacol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Zhijian,Zhou, Yunlong,Lu, Jinjian,et al. Caspase-8 preferentially senses the apoptosis-inducing action of NG-18, a gambogic acid derivative, in human leukemia HL-60 cells[J]. CANCER BIOLOGY & THERAPY,2007,6(5):691-696. |
| APA | Tao, Zhijian.,Zhou, Yunlong.,Lu, Jinjian.,Duan, Wenhu.,Qin, Yuxin.,...&Ding, Jian.(2007).Caspase-8 preferentially senses the apoptosis-inducing action of NG-18, a gambogic acid derivative, in human leukemia HL-60 cells.CANCER BIOLOGY & THERAPY,6(5),691-696. |
| MLA | Tao, Zhijian,et al."Caspase-8 preferentially senses the apoptosis-inducing action of NG-18, a gambogic acid derivative, in human leukemia HL-60 cells".CANCER BIOLOGY & THERAPY 6.5(2007):691-696. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。