Protein tyrosine phosphatase 1B regulates TGF beta 1-induced Smad2 activation through PI3 kinase-dependent pathway
文献类型：期刊论文
| 作者 | Sun, Tao; Ye, Fei; Ding, Hong ; Chen, Kaixian; Jiang, Hualiang; Shen, Xu
|
| 刊名 | CYTOKINE
 |
| 出版日期 | 2006-07 |
| 卷号 | 35期号:1-2页码:88-94 |
| 关键词 | TGF beta 1 Smad2 protein tyrosine phosphatase 1B (PTP1B) PI3K/AKT IN Cell Analyzer |
| ISSN号 | 1043-4666 |
| DOI | 10.1016/j.cyto.2006.07.013 |
| 文献子类 | Article |
| 英文摘要 | Insulin is known to modulate transforming growth factor-beta (TGF beta) signaling. In this report, by using the IN Cell Analyzer 1000, the fluorescence cell imaging instrument, we demonstrated that protein tyrosine phosphatase 1B (PTP1B) could regulate TGF beta 1-induced Smad2 activation in a PI3 kinase-dependent manner. By using the CHO cells stably expressing EGFP-Smad2, we showed that TGF beta 1 effectively stimulated Smad2 nuclear translocation in CHO cells. When pretreated with insulin, TGF beta 1-induced Smad2 nuclear entry was dramatically decreased. Furthermore, both the PI3K inhibitor LY294002 and the dominant negative AKT (DN-AKT) abolished the inhibitory effects of insulin, demonstrating that the inhibition of Smad2 activation by insulin was PI3K/AKT dependent. Since PTP1B negatively modulates insulin: signaling, we further addressed the effects of PTP1B on insulin -mediated inhibition of Smad2 activation. Our data showed that overexpression of PTP1B effectively attenuated insulin-induced inhibition of Smad2 stimulation. Moreover, the PTP1B inhibitor, 3-(3,5-dibromo-4-hydroxy-benzoyl)-2-ethyl-benzofuran-6-sulfonicacid-(4-(thiazo1-2-ylsulfamyl)-phenyl)-amide (Compound-2), recovered insulin inhibition of Smad2 activation. In conclusion, our data revealed the insulin inhibitory effects on TGF beta 1-induced Smad2 activation and the regulation role of PTP1B in the inhibition events. (c) 2006 Elseviei Ltd. All rights reserved. |
| WOS关键词 | GROWTH-FACTOR-BETA ; TGF-BETA ; PHOSPHATIDYLINOSITOL 3-KINASE ; INSULIN SENSITIVITY ; INDUCED APOPTOSIS ; AKT ; RECEPTOR ; OBESITY ; TARGET ; PHOSPHORYLATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000241494600012 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273554]  |
| 专题 | 药物发现与设计中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药理学第三研究室 |
| 通讯作者 | Jiang, Hualiang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res,Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Tao,Ye, Fei,Ding, Hong,et al. Protein tyrosine phosphatase 1B regulates TGF beta 1-induced Smad2 activation through PI3 kinase-dependent pathway[J]. CYTOKINE,2006,35(1-2):88-94. |
| APA | Sun, Tao,Ye, Fei,Ding, Hong,Chen, Kaixian,Jiang, Hualiang,&Shen, Xu.(2006).Protein tyrosine phosphatase 1B regulates TGF beta 1-induced Smad2 activation through PI3 kinase-dependent pathway.CYTOKINE,35(1-2),88-94. |
| MLA | Sun, Tao,et al."Protein tyrosine phosphatase 1B regulates TGF beta 1-induced Smad2 activation through PI3 kinase-dependent pathway".CYTOKINE 35.1-2(2006):88-94. |
入库方式： OAI收割
来源：上海药物研究所
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