H2O2 Inhibits ABA-Signaling Protein Phosphatase HAB1
文献类型：期刊论文
| 作者 | Sridharamurthy, Madhuri1,2,3; Kovach, Amanda1,2; Zhao, Yang4; Zhu, Jian-Kang4; Xu, H. Eric1,2,5 ; Swaminathan, Kunchithapadam3; Melcher, Karsten1,2
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| 刊名 | PLOS ONE
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| 出版日期 | 2014-12-02 |
| 卷号 | 9期号:12 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0113643 |
| 文献子类 | Article |
| 英文摘要 | Due to its ability to be rapidly generated and propagated over long distances, H2O2 is an important second messenger for biotic and abiotic stress signaling in plants. In response to low water potential and high salt concentrations sensed in the roots of plants, the stress hormone abscisic acid (ABA) activates NADPH oxidase to generate H2O2, which is propagated in guard cells in leaves to induce stomatal closure and prevent water loss from transpiration. Using a reconstituted system, we demonstrate that H2O2 reversibly prevents the protein phosphatase HAB1, a key component of the core ABA-signaling pathway, from inhibiting its main target in guard cells, SnRK2.6/OST1 kinase. We have identified HAB1 C186 and C274 as H2O2-sensitive thiols and demonstrate that their oxidation inhibits both HAB1 catalytic activity and its ability to physically associate with SnRK2.6 by formation of intermolecular dimers. |
| WOS关键词 | OXYGEN SPECIES PRODUCTION ; ABSCISIC-ACID ACTIVATION ; INDUCED STOMATAL CLOSURE ; ARABIDOPSIS GUARD-CELLS ; HYDROGEN-PEROXIDE ; REVERSIBLE INACTIVATION ; NEGATIVE REGULATOR ; OXIDATIVE STRESS ; ANION CHANNEL ; WATER-STRESS |
| 资助项目 | Jay and Betty Van Andel Foundation[00000000] ; National Institutes of Health from the Academic Research Fund of Singapore[R01 GM102545] ; National University of Singapore[00000000] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000345869700040 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276809]  |
| 专题 | 药物靶标结构与功能中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Swaminathan, Kunchithapadam |
| 作者单位 | 1.Van Andel Res Inst, Lab Struct Sci Struct Biol, Grand Rapids, MI USA; 2.Van Andel Res Inst, Biochem Lab, Grand Rapids, MI USA; 3.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore; 4.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47906 USA; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci,Ctr Struct & Funct Drug Ta, State Key Lab Drug Res,VARI SIMM Ctr, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sridharamurthy, Madhuri,Kovach, Amanda,Zhao, Yang,et al. H2O2 Inhibits ABA-Signaling Protein Phosphatase HAB1[J]. PLOS ONE,2014,9(12). |
| APA | Sridharamurthy, Madhuri.,Kovach, Amanda.,Zhao, Yang.,Zhu, Jian-Kang.,Xu, H. Eric.,...&Melcher, Karsten.(2014).H2O2 Inhibits ABA-Signaling Protein Phosphatase HAB1.PLOS ONE,9(12). |
| MLA | Sridharamurthy, Madhuri,et al."H2O2 Inhibits ABA-Signaling Protein Phosphatase HAB1".PLOS ONE 9.12(2014). |
入库方式： OAI收割
来源：上海药物研究所
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