Inhibitors of HIV-1 Integrase-Human LEDGF/p75 Interaction Identified from Natural Products via Virtual Screening
文献类型：期刊论文
| 作者 | Hu Guoping2; Li Xi2; Li Yaozong2; Sun Xianqiang2; Liu Guixia2; Li Weihua2; Huang Jin2; Shen Xu1 ; Tang Yun2
|
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2012-12 |
| 卷号 | 30期号:12页码:2752-2758 |
| 关键词 | natural products virtual screening protein-protein interaction HIV-1 integrase human LEDGF p75 protein |
| ISSN号 | 1001-604X |
| DOI | 10.1002/cjoc.201200897 |
| 文献子类 | Article |
| 英文摘要 | HIV-1 integrase (IN)-mediated integration of viral DNA into the host chromosome is an essential step in the virus life cycle. Human lens epithelium-derived growth factor (LEDGF/p75) has been found to function as a cellular cofactor in this process. The LEDGF/p75-IN interaction hence represents an attractive target for anti-HIV therapy. In this study, natural products were virtually screened against the LEDGF/p75 binding pocket of HIV-1 IN. 24 compounds were selected and obtained from the National Compound Resource Center of China. AlphaScreen assays characterized 8 of these 24 natural products as potent LEDGF/p75-IN interaction inhibitors. The active compounds whose IC50 values ranged from 0.56 to 14.55 mu mol/L could be used as lead compounds for further investigation. This work confirmed that natural products are valuable resources for antiviral drug discovery. |
| WOS关键词 | SMALL-MOLECULE INHIBITORS ; PROTEIN INTERACTIONS ; CELLULAR COFACTORS ; BINDING-SITE ; PHARMACOPHORE ; DERIVATIVES ; DISCOVERY ; INSIGHTS ; ACID |
| 资助项目 | Program for New Century Excellent Talents in University[NCET-08-0774] ; Innovation Program of Shanghai Municipal Education Commission[10ZZ41] ; National Natural Science Foundation of China[21072059] ; Shanghai Committee of Science and Technology[11DZ2260600] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4713293 |
| WOS记录号 | WOS:000312942500006 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277852]  |
| 专题 | 药理学第三研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Huang Jin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Hu Guoping,Li Xi,Li Yaozong,et al. Inhibitors of HIV-1 Integrase-Human LEDGF/p75 Interaction Identified from Natural Products via Virtual Screening[J]. CHINESE JOURNAL OF CHEMISTRY,2012,30(12):2752-2758. |
| APA | Hu Guoping.,Li Xi.,Li Yaozong.,Sun Xianqiang.,Liu Guixia.,...&Tang Yun.(2012).Inhibitors of HIV-1 Integrase-Human LEDGF/p75 Interaction Identified from Natural Products via Virtual Screening.CHINESE JOURNAL OF CHEMISTRY,30(12),2752-2758. |
| MLA | Hu Guoping,et al."Inhibitors of HIV-1 Integrase-Human LEDGF/p75 Interaction Identified from Natural Products via Virtual Screening".CHINESE JOURNAL OF CHEMISTRY 30.12(2012):2752-2758. |
入库方式： OAI收割
来源：上海药物研究所
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