Abscisic Acid Signaling: Thermal Stability Shift Assays as Tool to Analyze Hormone Perception and Signal Transduction
文献类型：期刊论文
| 作者 | Soon, Fen-Fen1,2; Suino-Powell, Kelly M.1; Li, Jun1,2 ; Yong, Eu-Leong2; Xu, H. Eric1,3; Melcher, Karsten1
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| 刊名 | PLOS ONE
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| 出版日期 | 2012-10-24 |
| 卷号 | 7期号:10 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0047857 |
| 文献子类 | Article |
| 英文摘要 | Abscisic acid (ABA) is a plant hormone that plays important roles in growth and development. ABA is also the central regulator to protect plants against abiotic stresses, such as drought, high salinity, and adverse temperatures, and ABA signaling is therefore a promising biotechnological target for the generation of crops with increased stress resistance. Recently, a core signal transduction pathway has been established, in which ABA receptors, type 2C protein phosphatases, and AMPK-related protein kinases control the regulation of transcription factors, ion channels, and enzymes. Here we use a simple protein thermal stability shift assay to independently validate key aspects of this pathway and to demonstrate the usefulness of this technique to detect and characterize very weak (Kd >= 50 mu M) interactions between receptors and physiological and synthetic agonists, to determine and analyze protein-protein interactions, and to screen small molecule inhibitors. |
| WOS关键词 | PROMOTE PROTEIN STABILITY ; STRUCTURAL BASIS ; TITRATION CALORIMETRY ; SCREENING METHODS ; SNRK2 KINASES ; PYL PROTEINS ; ABA ; ARABIDOPSIS ; IDENTIFICATION ; ACTIVATION |
| 资助项目 | Jay and Betty Van Andel Foundation and Amway (China) Limited (HEX)[00000000] ; National Institutes of Health (HEX)[00000000] ; Singapore Biomedical Research Council (ELY)[00000000] ; NUS Graduate School for Integrative Sciences & Engineering (NGS)[00000000] ; Singapore National Medical Research Council[R-174-000-137-275] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000310310200094 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277905]  |
| 专题 | 药物靶标结构与功能中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Xu, H. Eric |
| 作者单位 | 1.Van Andel Res Inst, NE, Lab Struct Sci, Grand Rapids, MI USA; 2.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Obstet & Gynecol, Natl Univ Hosp, Singapore 117595, Singapore; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, State Key Lab Drug Res,VARISIMM Ctr,Ctr Struct &, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Soon, Fen-Fen,Suino-Powell, Kelly M.,Li, Jun,et al. Abscisic Acid Signaling: Thermal Stability Shift Assays as Tool to Analyze Hormone Perception and Signal Transduction[J]. PLOS ONE,2012,7(10). |
| APA | Soon, Fen-Fen,Suino-Powell, Kelly M.,Li, Jun,Yong, Eu-Leong,Xu, H. Eric,&Melcher, Karsten.(2012).Abscisic Acid Signaling: Thermal Stability Shift Assays as Tool to Analyze Hormone Perception and Signal Transduction.PLOS ONE,7(10). |
| MLA | Soon, Fen-Fen,et al."Abscisic Acid Signaling: Thermal Stability Shift Assays as Tool to Analyze Hormone Perception and Signal Transduction".PLOS ONE 7.10(2012). |
入库方式： OAI收割
来源：上海药物研究所
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