Succinate-acetate permease from Citrobacter koseri is an anion channel that unidirectionally translocates acetate
文献类型：期刊论文
| 作者 | Qiu, Biao8,9; Xia, Bingqing6,7; Zhou, Qingtong1; Lu, Yan8,9; He, Miaomiao8,9; Hasegawa, Kazuya2; Ma, Zhiqiang3; Zhang, Fengyu4; Gu, Lichuan4; Mao, Qionglei6,7 |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2018-06 |
| 卷号 | 28期号:6页码:644-654 |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/s41422-018-0032-8 |
| 文献子类 | Article |
| 英文摘要 | Acetate is an important metabolite in metabolism and cell signaling. Succinate-Acetate Permease (SatP) superfamily proteins are known to be responsible for acetate transport across membranes, but the nature of this transport remains unknown. Here, we show that the SatP homolog from Citrobacter koseri (SatP_Ck) is an anion channel that can unidirectionally translocate acetate at rates of the order of similar to 10(7) ions/s. Crystal structures of SatP_Ck in complex with multiple acetates at 1.8 angstrom reveal that the acetate pathway consists of four acetate-binding sites aligned in a single file that are interrupted by three hydrophobic constrictions. The bound acetates at the four sites are each orientated differently. The acetate at the cytoplasmic vestibule is partially dehydrated, whereas those in the main pore body are fully dehydrated. Aromatic residues within the substrate pathway may coordinate translocation of acetates via anion-p interactions. SatP_Ck reveals a new type of selective anion channel and provides a structural and functional template for understanding organic anion transport. |
| WOS关键词 | CHAIN FATTY-ACIDS ; FORMATE CHANNEL ; YARROWIA-LIPOLYTICA ; ESCHERICHIA-COLI ; CHLORIDE CHANNEL ; MOLECULAR-BASIS ; ACETIC-ACID ; PROTEIN ; ACETYLATION ; TRANSPORTER |
| 资助项目 | Ministry of Science and Technology of China[2017YFA0504800] ; National Science and Technology Major Projects for "Major New Drugs Innovation and Development"[2018ZX09711003-003-003] ; National Key Scientific Instrument and Equipment Development Program[2012YQ03026010] ; Joint Fund of the National Natural Science Foundation of China[00000000] ; Israel Science Foundation Research Program[8146114802] ; China Post-Doctoral Science Foundation[2016M600344] ; Shanghai Municipal Government[00000000] ; ShanghaiTech University[00000000] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6266442 |
| WOS记录号 | WOS:000434816300007 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279729]  |
| 专题 | 神经药理学研究国际科学家工作站 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Gao, Zhaobing; Liao, Jun |
| 作者单位 | 1.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China; 2.Japan Synchrotron Radiat Res Inst, Prot Crystal Anal Div, Sayo, Hyogo 6795198, Japan; 3.South China Univ Technol, Key Lab Funct Mol Engn Guangdong Prov, Sch Chem & Chem Engn, Guangzhou 510640, Guangdong, Peoples R China; 4.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China; 5.Wuxi Biortus Biosci Co Ltd, Wuxi 214437, Jiangsu, Peoples R China; 6.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 7.Chinese Acad Sci, CAS Key Lab Receptor Res, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 8.Chinese Acad Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China; 9.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Qiu, Biao,Xia, Bingqing,Zhou, Qingtong,et al. Succinate-acetate permease from Citrobacter koseri is an anion channel that unidirectionally translocates acetate[J]. CELL RESEARCH,2018,28(6):644-654. |
| APA | Qiu, Biao.,Xia, Bingqing.,Zhou, Qingtong.,Lu, Yan.,He, Miaomiao.,...&Liao, Jun.(2018).Succinate-acetate permease from Citrobacter koseri is an anion channel that unidirectionally translocates acetate.CELL RESEARCH,28(6),644-654. |
| MLA | Qiu, Biao,et al."Succinate-acetate permease from Citrobacter koseri is an anion channel that unidirectionally translocates acetate".CELL RESEARCH 28.6(2018):644-654. |
入库方式： OAI收割
来源：上海药物研究所
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