Preparation of Methylated Products of A-type Procyanidin Trimers in Cinnamon Bark and Their Protective Effects on Pancreatic beta-Cell
文献类型：期刊论文
| 作者 | Chen, Lu2; Chen, Liang2; Wang, Ting1; Yuan, Pulong2; Chen, Kaixian1,2 ; Jia, Qi2; Wang, Heyao1; Li, Yiming2
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| 刊名 | JOURNAL OF FOOD SCIENCE
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| 出版日期 | 2016-05 |
| 卷号 | 81期号:5页码:C1062-C1069 |
| 关键词 | A-type procyanidin trimer antidiabetic effects cinnamon cinnamtannin-1 methylation |
| ISSN号 | 0022-1147 |
| DOI | 10.1111/1750-3841.13294 |
| 文献子类 | Article |
| 英文摘要 | Polyphenols are partial metabolized to methylated conjugations in vivo, and then could modify bioavailability and bioactivity related to the uptake of parent compounds. Our previous studies have found that the antidiabetic effects of cinnamon barks are mainly related to polyphenol components, particularly A-type procyanidin trimer cinnamtannin-1 (CT1). It is necessary to understand the antidiabetic activity of methylations of CT1, nevertheless, sufficient amounts of methylated CT1 are difficult to obtain from metabolites in vivo. In this study, O-methyl derivatives of CT1 were prepared through one-pot methyl iodide reaction and isolation via column chromatography and RP-HPLC semipreparation. The structures of O-methyl substituents were determined through NMR (Nuclear Magnetic Resonance) and HPLC-ESI-MS (High-performance liquid chromatography-electrospray ionization-mass spectrometry). Five purified O-methyl substituents and 2 isomers of CT1 were obtained. Their protective effects on a palmitic acid-induced pancreatic beta-cell apoptosis model were then evaluated. Results showed that the protective effects on pancreatic beta-cell of O-methyl substituents were weaker than those of CT1. The results suggested that the methylation of catechol groups could be a relevant factor contributing to the decline of protective effects on pancreatic beta-cell of CT1 via obstructing quinone intermediate formation and affecting antioxidant abilities. The antidiabetic effects of O-methyl derivatives of CT1 should be further determined by other antidiabetic models. |
| WOS关键词 | OXIDATIVE STRESS ; INSULIN-SECRETION ; CATECHIN ; PROANTHOCYANIDINS ; ANTIOXIDANTS ; MACROPHAGES ; CONVERSION ; ANALOGS ; TEA |
| 资助项目 | Natural Science Foundation of Shanghai, China[15ZR1441200] ; Eastern Scholar Tracking Program of Shanghai Municipal Education Commission[2012-90] ; "Shanghai E-Research Institute of Bioactive Constituent in TCM" Plan[00000000] |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000378917300003 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276045]  |
| 专题 | 药理学第三研究室 天然药物化学研究室 |
| 通讯作者 | Wang, Heyao; Li, Yiming |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Sch Pharm, 1200 Cailun Rd, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Chen, Lu,Chen, Liang,Wang, Ting,et al. Preparation of Methylated Products of A-type Procyanidin Trimers in Cinnamon Bark and Their Protective Effects on Pancreatic beta-Cell[J]. JOURNAL OF FOOD SCIENCE,2016,81(5):C1062-C1069. |
| APA | Chen, Lu.,Chen, Liang.,Wang, Ting.,Yuan, Pulong.,Chen, Kaixian.,...&Li, Yiming.(2016).Preparation of Methylated Products of A-type Procyanidin Trimers in Cinnamon Bark and Their Protective Effects on Pancreatic beta-Cell.JOURNAL OF FOOD SCIENCE,81(5),C1062-C1069. |
| MLA | Chen, Lu,et al."Preparation of Methylated Products of A-type Procyanidin Trimers in Cinnamon Bark and Their Protective Effects on Pancreatic beta-Cell".JOURNAL OF FOOD SCIENCE 81.5(2016):C1062-C1069. |
入库方式： OAI收割
来源：上海药物研究所
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