Solution structure and backbone dynamics of human Raf-1 kinase inhibitor protein
文献类型：期刊论文
| 作者 | Guo, Chenyun1; Yi, Cuiying2; Peng, Yu2; Wen, Yi2; Lin, Donghai1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2013-08-16 |
| 卷号 | 438期号:1页码:129-132 |
| 关键词 | Human Raf-1 kinase inhibitor protein (hRKIP) Solution structure Ligand-binding pocket NMR Model-free analysis Protein dynamics |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2013.07.039 |
| 文献子类 | Article |
| 英文摘要 | Human Raf-1 kinase inhibitor protein (hRKIP) is a small multi-functional protein of 187 residues. It contains a conserved pocket, which binds a wide range of ligands from various small molecules to distinct proteins. To provide a structural basis for the ligand diversity of RKIP, we herein determined the solution structure of hRKIP, and analyzed its structural dynamics. In solution, hRKIP mainly comprises two anti-parallel beta sheets, two alpha helices and two 3(10) helices. NMR dynamic analysis reveals that the overall structure of hRKIP is rigid, but its C-terminal helix which is close to the ligand-binding site is mobile. In addition, residues around the ligand-binding pocket exhibit significant conformational exchange on the mu s-ms timescale. Conformational flexibility may allow the ligand-binding pocket and the C-terminal helix to adopt various conformations to interact with different substrates. This work may shed light on the underlying molecular mechanisms of how hRKIP recognizes and binds diverse substrate ligands. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS关键词 | CANCER METASTASIS ; CHEMICAL-SHIFT ; BOVINE BRAIN ; NMR ; SUPPRESSION ; EXPRESSION ; BINDING ; RKIP |
| 资助项目 | Chinese National Science Foundation[30900233] ; Chinese National Science Foundation[31170717] ; Chinese National Science Foundation[91129713] ; Natural Science Foundation of Fujian Province[2011J01246] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000323467100022 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277505]  |
| 专题 | 分析化学研究室 药物靶标结构与功能中心 |
| 通讯作者 | Lin, Donghai |
| 作者单位 | 1.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen 361005, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, NMR Lab, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Chenyun,Yi, Cuiying,Peng, Yu,et al. Solution structure and backbone dynamics of human Raf-1 kinase inhibitor protein[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,438(1):129-132. |
| APA | Guo, Chenyun,Yi, Cuiying,Peng, Yu,Wen, Yi,&Lin, Donghai.(2013).Solution structure and backbone dynamics of human Raf-1 kinase inhibitor protein.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,438(1),129-132. |
| MLA | Guo, Chenyun,et al."Solution structure and backbone dynamics of human Raf-1 kinase inhibitor protein".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 438.1(2013):129-132. |
入库方式： OAI收割
来源：上海药物研究所
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