Optimization of the culture condition for an antitumor bacterium Serratia proteamacula 657 and identification of the active compounds
文献类型：期刊论文
| 作者 | Miao, Li1,2; Wang, Xueling1; Jiang, Wei1; Yang, Shengping2; Zhou, Huiru1; Zhai, Youpeng1; Zhou, Xiaojian1; Dong, Kunming1 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2013-05 |
| 卷号 | 29期号:5页码:855-863 |
| 关键词 | Antitumor activity Serratia proteamacula Optimization of culture conditions Prodigiosin |
| ISSN号 | 0959-3993 |
| DOI | 10.1007/s11274-012-1240-x |
| 文献子类 | Article |
| 英文摘要 | Exploration of novel active anti-tumor compounds from marine microbes for pharmaceutical applications has been a continuously hot spot in natural product research. Bacterial growth and metabolites may greatly vary under different culture conditions. In this study, the effects of different culture conditions and medium components on the growth and bioactive metabolites of Serratia proteamacula 657, an anti-tumor bacterium found in our previous study, were investigated. The results showed that lower temperature, weak acidic condition and solid fermentation favored the bacterial growth and the production of active compounds. Four components in the culture medium, NaCl, peptone, yeast extract and MgSO4, were found important to the bacterial growth and active compounds production in medium optimization. Under the optimized condition of solid state fermentation at pH 6.0-7.0, 23-25 A degrees C, with the MgSO4-free medium containing 10.0 g/L peptone, 1.0 g/L yeast extract and 19.45 g/L NaCl, the antitumor activity of S. proteamacula 657 and the yield of crude extracts increased about 15 times and 6 times than the sample obtained in the original liquid fermentation, respectively. The active components in the metabolites of S. proteamacula 657 were identified as a homolog of prodigiosin, a red bacterial pigment, based on the analysis of the NMR and GC-MS. The bacterium S. proteamacula 657, which is adapted to lower temperature, produced prodigiosin-like pigments with highly antitumor activity, suggesting the bacterium is a potential new source for prodigiosin production. |
| WOS关键词 | MARINE NATURAL-PRODUCTS ; CELL LINES ; PRODIGIOSIN ; SUPPLEMENTATION |
| 资助项目 | National Natural Science Foundation of China[41006097] ; National Natural Science Foundation of China[41076097] ; National Natural Science Foundation of China[41271521] ; State Key Laboratory of Drug Research[SIMM1106KF-09] ; Key Laboratory of Marine Drug, Ministry of Education[KLMD(OUC)201203] ; Natural Science Foundation for college and university of Jiangsu Province[10KJB170013] ; Natural Science Foundation of Jiangsu Province[BK2012267] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000317174200010 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277644]  |
| 专题 | 天然药物化学研究室 |
| 通讯作者 | Dong, Kunming |
| 作者单位 | 1.Yangzhou Univ, Sch Environm Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, Li,Wang, Xueling,Jiang, Wei,et al. Optimization of the culture condition for an antitumor bacterium Serratia proteamacula 657 and identification of the active compounds[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(5):855-863. |
| APA | Miao, Li.,Wang, Xueling.,Jiang, Wei.,Yang, Shengping.,Zhou, Huiru.,...&Dong, Kunming.(2013).Optimization of the culture condition for an antitumor bacterium Serratia proteamacula 657 and identification of the active compounds.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(5),855-863. |
| MLA | Miao, Li,et al."Optimization of the culture condition for an antitumor bacterium Serratia proteamacula 657 and identification of the active compounds".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.5(2013):855-863. |
入库方式： OAI收割
来源：上海药物研究所
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